Liu Qianqian, Bai Na, Duan Shipeng, Shen Yanmei, Zhu Lirong, Yang Jinkui
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, School of Life Science, Yunnan University, Kunming 650032, China.
School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Microorganisms. 2024 Mar 19;12(3):615. doi: 10.3390/microorganisms12030615.
, a widespread nematode-trapping fungus which can produce conidia for asexual reproduction and form trapping devices (traps) to catch nematodes. However, little is known about the sporulation mechanism of . This research characterized the functions and regulatory roles of the upstream spore-producing regulatory genes, and , in . Our analysis showed that and interacted with each other. Meanwhile, the gene was downregulated in the Δ mutant strain, indicating that positively regulates . Loss of the and genes led to shorter hyphae and more septa, and the Δ strain responded to heat and chemical stresses. Surprisingly, the number of nuclei was increased in the mycelia but reduced in the conidia of the Δ and Δ mutants. In addition, after nematode induction, the number and volume of vacuoles were remarkably increased in the Δ and Δ mutant strains. The abundance of metabolites was markedly decreased in the Δ and Δ mutant strains. Collectively, the and genes play critical roles in mycelial development, and they are also involved in vacuole assembly, the stress response, and secondary metabolism. Our study provides distinct insights into the regulatory mechanism of sporulation in nematode-trapping fungi.
是一种广泛存在的捕食线虫真菌，它能够产生用于无性繁殖的分生孢子，并形成捕捉装置（陷阱）来捕获线虫。然而，关于的孢子形成机制知之甚少。本研究对上游产孢调控基因和在中的功能及调控作用进行了表征。我们的分析表明和相互作用。同时，基因在Δ突变菌株中表达下调，表明正向调控。和基因的缺失导致菌丝变短且隔膜增多，并且Δ菌株对热和化学胁迫有反应。令人惊讶的是，Δ和Δ突变体的菌丝体中细胞核数量增加，但分生孢子中细胞核数量减少。此外，线虫诱导后，Δ和Δ突变菌株中液泡的数量和体积显著增加。Δ和Δ突变菌株中代谢物的丰度显著降低。总的来说，和基因在菌丝体发育中起关键作用，它们还参与液泡组装、应激反应和次级代谢。我们的研究为捕食线虫真菌孢子形成的调控机制提供了独特的见解。
