A株Δ-m (P353L)-SC19在小鼠模型中对2型和9型菌株攻击提供交叉保护。
A Strain Δ-m (P353L)-SC19 Provides Cross-Protection against Serotypes 2 and 9 Strain Challenges in a Mouse Model.
作者信息
Lu Xi, Xu Lei, Lin Lan, Zhou Liting, Dai Bingqian, Cui Shuyue, Zhang Anding
机构信息
State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
出版信息
Vaccines (Basel). 2024 Mar 8;12(3):283. doi: 10.3390/vaccines12030283.
is an important zoonotic pathogen that mainly causes meningitis, septicemia, and arthritis. Due to the limited cross-protection between numerous serotypes, the existing inactive vaccines in clinical use fail to offer sufficient protection. In this study, a gene deletion-attenuated strain Δ-m (P353L)-SC-19 was constructed by deleting and genes from the epidemic strain SC-19 with a mutation of SLY (P353L). The safety of Δ-m (P353L)-SC-19 was confirmed in both in vitro and in vivo experiments. We further demonstrated that immunization with Δ-m (P353L)-SC-19 induced significant cellular immunity and humoral immunity in mice and protected against infections caused by type 2 strain SC-19 (100% protection) and type 9 strain S29 (50% protection), while also preventing meningitis induced by S29. This study highlights the potential of using CPS-deficient strains to achieve cross-protection against different serotypes and develop a promising universal live vaccine.
是一种重要的人畜共患病原体，主要引起脑膜炎、败血症和关节炎。由于众多血清型之间的交叉保护有限，目前临床使用的灭活疫苗未能提供足够的保护。在本研究中，通过从流行菌株SC-19中删除 和 基因，并使SLY发生S353L突变，构建了基因缺失减毒株Δ-m (P353L)-SC-19。Δ-m (P353L)-SC-19的安全性在体外和体内实验中均得到证实。我们进一步证明，用Δ-m (P353L)-SC-19免疫可在小鼠体内诱导显著的细胞免疫和体液免疫，并预防由2型菌株SC-19（100%保护）和9型菌株S29（50%保护）引起的感染，同时还能预防S29诱导的脑膜炎。本研究突出了使用缺乏荚膜多糖的菌株实现针对不同血清型的交叉保护并开发有前景的通用活疫苗的潜力。
Zotero 插件