Silswal Akshay, P Kavyashree, Koner Apurba Lal
Bionanotechnology Lab, Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal 462066, Madhya Pradesh India.
ACS Omega. 2024 Mar 11;9(12):13494-13508. doi: 10.1021/acsomega.3c08606. eCollection 2024 Mar 26.
Metal ions are indispensable and play an important role in living systems. Metal ions coordinated to metalloenzymes pocket activate the bound substrate and labile metal ions maintaining the ionic balance. The amount of metal ions present in various subcellular compartments of the cells is highly regulated for maintaining cellular homeostasis. An imbalance in the metal ion concentration is related to several diseases and results in serious pathological conditions. Mostly the internalized metal ions are processed in the lysosomal compartment of the cell. A delicate regulation of metal ions in the lysosomal compartment can modulate the lysosomal pH and inhibit hydrolytic enzymes, which ultimately causes lysosomal storage disorders. In the past decade, the understanding and regulation of lysosomal metal ions based on fluorometric methods have gained significant attention. In this review, we have comprehensively summarized the development of various fluorescent reporters over the past five years for a selective and sensitive estimation of lysosomal metal ion concentration. We believe this consolidated and timely review will help researchers working in the areas associated with lysosomal metal ions.
金属离子在生命系统中不可或缺且发挥着重要作用。与金属酶口袋配位的金属离子激活结合的底物，而不稳定的金属离子维持离子平衡。细胞各种亚细胞区室中存在的金属离子数量受到严格调控以维持细胞内稳态。金属离子浓度失衡与多种疾病相关，并导致严重的病理状况。内化的金属离子大多在细胞的溶酶体区室中进行处理。溶酶体区室中金属离子的精细调控可调节溶酶体pH并抑制水解酶，最终导致溶酶体贮积症。在过去十年中，基于荧光法对溶酶体金属离子的理解和调控受到了广泛关注。在本综述中，我们全面总结了过去五年中各种荧光报告分子的发展，用于选择性和灵敏地估计溶酶体金属离子浓度。我们相信这一全面且及时的综述将有助于从事与溶酶体金属离子相关领域研究的人员。
