Zuo Runan, Kong Lingqing, Pang Wanjun, Jiang Shanxiang
Animal-derived food safety innovation team, College of Animal Science and Technology, Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei, Anhui 230036, PR China.
Engineering Center of Innovative Veterinary Drugs, Center for Veterinary Drug Research and Evaluation, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
Int J Pharm X. 2024 Mar 26;7:100241. doi: 10.1016/j.ijpx.2024.100241. eCollection 2024 Jun.
In female dogs, the highest morbidity and mortality rates cancer are the result of mammary adenocarcinoma, which presents with metastases in the lung. Other than early surgical removal, however, no special methods are available to treat mammary adenocarcinoma. Because human breast cancer and canine mammary carcinoma share clinical characteristics and heterogeneity, the canine model is a suitable spontaneous tumor model for breast cancer in humans. In this study, the physical swelling method was used to prepare halofuginone-loaded D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) polymer micelles nano-thermosensitive hydrogels (HTPM-gel). Furthermore, HTPM-gel was investigated via characterization, morphology, properties such as swelling experiment and release with reflecting its splendid nature. Moreover, HTPM-gel was further examined its capability to anti-proliferation, anti-migration, and anti-invasion. Ultimately, HTPM-gel was investigated for its anticancer activity in the post-operative metastatic and angiogenic canine mammary carcinoma. HTPM-gel presented spherical under transmission electron microscope (TEM) and represented grid structure under scanning electron microscope (SEM), with hydrodynamic diameter (HD) of 20.25 ± 2.5 nm and potential (ZP) of 15.10 ± 1.82 mV. Additionally, HTPM-gel own excellent properties comprised of pH-dependent swelling behavior, sustained release behavior. To impede the migration, invasion, and proliferation of CMT-U27 cells, we tested the efficacy of HTPM-gel. Evaluation of anti-tumor efficacy demonstrates HTPM-gel exhibit a splendid anti-metastasis and anti-angiogenic ability, with exhibiting ideal biocompatibility. Notably, HTPM-gel also inhibited the scar formation in the healing process after surgery. In summary, HTPM-gel exhibited anti-metastasis and anti-angiogenic and scar repair features. According to the results of this study, HTPM-gel has encouraging clinical potential to treat tumors with multifunctional hydrogel.
在雌性犬中，癌症的最高发病率和死亡率是乳腺腺癌导致的，乳腺腺癌会出现肺转移。然而，除了早期手术切除外，没有其他特殊方法可用于治疗乳腺腺癌。由于人类乳腺癌和犬类乳腺癌具有共同的临床特征和异质性，犬类模型是人类乳腺癌合适的自发肿瘤模型。在本研究中，采用物理溶胀法制备了负载卤夫酮的聚乙二醇1000琥珀酸维生素E（TPGS）聚合物胶束纳米热敏水凝胶（HTPM-凝胶）。此外，通过表征、形态、溶胀实验和释放等性质对HTPM-凝胶进行了研究，以反映其优异特性。此外，还进一步研究了HTPM-凝胶的抗增殖、抗迁移和抗侵袭能力。最终，研究了HTPM-凝胶在术后转移性和血管生成性犬乳腺腺癌中的抗癌活性。HTPM-凝胶在透射电子显微镜（TEM）下呈球形，在扫描电子显微镜（SEM）下呈网格结构，流体动力学直径（HD）为20.25±2.5nm，电位（ZP）为15.10±1.82mV。此外，HTPM-凝胶具有优异的性质，包括pH依赖性溶胀行为、缓释行为。为了抑制CMT-U27细胞的迁移、侵袭和增殖，我们测试了HTPM-凝胶的功效。抗肿瘤功效评估表明，HTPM-凝胶具有出色的抗转移和抗血管生成能力，并表现出理想的生物相容性。值得注意的是，HTPM-凝胶还抑制了手术后愈合过程中的瘢痕形成。总之，HTPM-凝胶具有抗转移、抗血管生成和瘢痕修复特性。根据本研究结果，HTPM-凝胶作为多功能水凝胶治疗肿瘤具有令人鼓舞的临床潜力。
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