新种和新种，源自冷藏牛肉和鸡胸肉。

sp. nov. and sp. nov., originating from chilled beef and chicken breast.

机构信息

Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Department of Safety and Quality of Meat, E.-C.-Baumann Straße 20, D-95326 Kulmbach, Germany.

Center for Biotechnology - CeBiTec, Bielefeld University, Universitätsstraße 27, D-33615 Bielefeld, Germany.

出版信息

Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006293.

DOI:10.1099/ijsem.0.006293

PMID:38587505

Abstract

By investigating wet and dry age-related ripening of beef, strains V3/3/4/13 and V3/K/3/5 were isolated. Strain V3/3/4/13 exhibited more than 99 % 16S rRNA gene-based similarity to and other members of this group, while isolate V3/K/3/5 was very close to and a number of relatives within the group. Additional comparisons of complete sequences and draft genomes allowed us to place isolate V3/3/4/13 close to DSM 26521. In the case of V3/K/3/5 the closest relative was DSM 11331. Average nucleotide identity (ANIb) and digital DNA-DNA hybridization (dDDH) values calculated from the draft genomes of V3/3/4/13 and DSM 26521 were 88.5 and 39.8 %, respectively. For V3/K/3/5 and its closest relative DSM 11331, the ANIb value was 95.1 % and the dDDH value was 60.7 %. The DNA G+C contents of V3/3/4/13 and V3/K/3/5 were 57.4 and 60.8 mol%, respectively. Predominant fatty acids were C, C ω7, C cyclo and summed feature C ω7/C iso 2OH. The main respiratory quinones were Q9, with minor proportions of Q8 and, in the case of V3/K/3/5, additional Q10. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and, in the case of V3/K/3/5, additional phosphatidylcholine. Based on the combined data, isolates V3/3/4/13 and V3/K/3/5 should be considered as representatives of two novel species. The type strain of the newly proposed sp. nov. is V3/3/4/13 (=DSM 113654=LMG 32520), a second strain belonging to the same species is FLM 004-28 (=DSM 113604=LMG 32521); the type strain for the newly proposed sp. nov. is V3/K/3/5 (=DSM 113573=LMG 32518) with a second isolate FLM 11 (=DSM 113572=LMG 32519).

摘要

通过研究牛肉的湿态和干态成熟相关老化，分离到了菌株 V3/3/4/13 和 V3/K/3/5。菌株 V3/3/4/13 的 16S rRNA 基因相似度超过 99%，与该组的其他成员以及其他成员非常相似，而分离株 V3/K/3/5 与及其许多亲缘关系密切的属内成员非常相似。对完整序列和草图基因组的进一步比较使我们能够将分离株 V3/3/4/13 与 DSM 26521 放在一起。对于 V3/K/3/5，最接近的亲缘关系是 DSM 11331。从 V3/3/4/13 和 DSM 26521 的草图基因组计算得出的平均核苷酸同一性 (ANIb) 和数字 DNA-DNA 杂交 (dDDH) 值分别为 88.5%和 39.8%。对于 V3/K/3/5 和其最接近的亲缘关系 DSM 11331，ANIb 值为 95.1%，dDDH 值为 60.7%。V3/3/4/13 和 V3/K/3/5 的 DNA G+C 含量分别为 57.4 mol%和 60.8 mol%。优势脂肪酸为 C、C ω7、C 环和总和特征 C ω7/C iso 2OH。主要呼吸醌为 Q9，Q8 的比例较小，而 V3/K/3/5 中还存在 Q10。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺，而 V3/K/3/5 中还存在额外的磷脂酰胆碱。基于综合数据，分离株 V3/3/4/13 和 V3/K/3/5 应被视为两个新型物种的代表。新提出的 sp. nov. 的模式株是 V3/3/4/13 (=DSM 113654=LMG 32520)，属于同一物种的第二个菌株是 FLM 004-28 (=DSM 113604=LMG 32521)；新提出的 sp. nov. 的模式株是 V3/K/3/5 (=DSM 113573=LMG 32518)，第二个分离株是 FLM 11 (=DSM 113572=LMG 32519)。