抗坏血酸作为炎症反应调节剂对抗。
Ascorbic acid as a modulator of inflammatory response against .
机构信息
Department of Environmental Engineering & Sciences Applied in Oral Health Graduate Program, São Paulo State University (Unesp), Institute of Science & Technology, São José dos Campos, São Paulo, 12245-000, Brazil.
Department of Biomedical & Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN 46202, USA.
出版信息
Future Microbiol. 2024;19(7):585-594. doi: 10.2217/fmb-2023-0188. Epub 2024 Apr 17.
To evaluate the behavior of oral keratinocytes in the presence of Vitamin C (Vit C) and its anti-inflammatory potential. Oral keratinocytes were initially exposed to 0.1-2.5 mM of Vit C and the metabolic activity and cell migration were evaluated using MTS assay and Ibidi culture inserts, respectively. After, the cells were challenged with and inflammatory markers were analyzed by qPCR. The treatment was not cytotoxic, and the highest concentrations increased the metabolic activity at 24 h. Vit C delayed the cell migration at 48 and 72 h. Interestingly, it downregulated the genes and . Vit C could be an interesting adjuvant to anti-fungal treatment due to its anti-inflammatory potential.
为了评估维生素 C（Vit C）存在时口腔角质细胞的行为及其抗炎潜力。首先将口腔角质细胞暴露于 0.1-2.5 mM 的 Vit C，分别使用 MTS 测定法和 Ibidi 培养插入物评估代谢活性和细胞迁移。然后，用 LPS 刺激细胞，并通过 qPCR 分析炎症标志物。该处理没有细胞毒性，并且最高浓度在 24 小时增加了代谢活性。Vit C 在 48 和 72 小时延迟了细胞迁移。有趣的是，它下调了 和 基因。由于其抗炎潜力，Vit C 可能是抗真菌治疗的一种有趣的辅助剂。
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