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密闭条件下生长牛气体通量及动物性能测试持续时间的测定

Determination of gas flux and animal performance test duration of growing cattle in confined conditions.

作者信息

Vargas Juan de J, Swenson Maya, Place Sara E

机构信息

Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523, USA.

出版信息

Transl Anim Sci. 2024 Apr 8;8:txae056. doi: 10.1093/tas/txae056. eCollection 2024.

Abstract

Data from three experiments was analyzed to determine the number of visits and days to assess gas flux (CH, CO, and O), dry matter intake (DMI), and average daily gain (ADG) from growing animals under confined conditions. In experiment 1, 213 animals (461 ± 91 kg initial body weight [BW]) were fed a backgrounding diet and evaluated for 60 d. In experiment 2, 169 steers (488 ± 37 kg initial BW) were fed a finishing diet and assessed for 70 d. In experiment 3, 64 steers (514 ± 42 kg initial BW) were fed a finishing diet and evaluated for 80 d. In each experiment, animals were placed in one pen with one Greenfeed and five SmartFeeds to collect gas flux and feed intake simultaneously. Gas flux was analyzed using data from 161 animals from the three experiments with 100 visits for 2 or more min or 3 or more min. Also, metabolic heat production (MHP) was estimated using the individual gas flux. Daily DMI was calculated as the daily feed intake corrected by the dry matter concentration. ADG was computed as the slope of the regression of the shrunk BW (96% BW) throughout each of the experimental periods. The mean gas flux and MHP were estimated for increasing or decreasing 5-visit intervals starting with the first or the last 5 visits and increasing or decreasing until the full 100-visit dataset was utilized, respectively. Intervals of DMI were estimated for increasing or decreasing 5-d intervals starting with the first or the last 5 d and increasing or decreasing until the end of the experimental period, respectively. Intervals of ADG were estimated for increasing or decreasing measurement period intervals until the end of the experimental period, respectively. Pearson and Spearman correlations were computed between the maximum visits or days and each shortened visit or day interval. The minimum number of visits and days was determined when correlations with the total visits were greater than 0.95. The results indicated that the minimum number of visits needed to quantify CO, O, and MHP accurately was 40, while CH was 60. A visitation length of 2 min or more or 3 min or more did not modify the gas flux determination. Thus, based on the average daily visitation in these experiments, gas flux data could be collected for 25 d. Additionally, the required days to determine DMI was 30, while ADG could not be assessed in a shorter than 60-d period.

摘要

分析了来自三个实验的数据,以确定在密闭条件下生长动物的气体通量(CH、CO和O)、干物质摄入量(DMI)和平均日增重(ADG)的访问次数和天数。在实验1中,213只动物(初始体重[BW]为461±91千克)被喂食育成期日粮,并评估60天。在实验2中,169头阉牛(初始体重为488±37千克)被喂食育肥期日粮,并评估70天。在实验3中,64头阉牛(初始体重为514±42千克)被喂食育肥期日粮,并评估80天。在每个实验中,将动物放置在一个装有一个Greenfeed和五个SmartFeeds的围栏中,以同时收集气体通量和采食量。使用来自三个实验的161只动物的数据进行气体通量分析,这些动物有100次持续2分钟或更长时间或3分钟或更长时间的访问。此外,使用个体气体通量估算代谢产热(MHP)。每日DMI通过干物质浓度校正后的每日采食量来计算。ADG计算为整个实验期间收缩体重(96%BW)回归的斜率。分别从第一次或最后5次访问开始,以5次访问为间隔增加或减少,直到使用完整的100次访问数据集,估算平均气体通量和MHP。分别从第一天或最后5天开始,以5天为间隔增加或减少,直到实验期结束,估算DMI的间隔。分别以测量期间间隔增加或减少,直到实验期结束,估算ADG的间隔。计算最大访问次数或天数与每个缩短的访问或天间隔之间的Pearson和Spearman相关性。当与总访问次数的相关性大于0.95时,确定最小访问次数和天数。结果表明,准确量化CO、O和MHP所需的最小访问次数为40次,而CH为60次。2分钟或更长时间或3分钟或更长时间的访问长度不会改变气体通量的测定。因此,根据这些实验中的平均每日访问次数,可以收集25天的气体通量数据。此外,确定DMI所需的天数为30天,而ADG在短于60天的时间段内无法评估。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7da/11025626/eecbdfced403/txae056_fig1.jpg

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