Hu Hanlin, Lu Jingyu, Xu Mingjin, Wang Jie, Zhang Yeling, Yang Shan, Wang Xiaomin, Wang Mengyuan, Xie Wenjie, Xu Wenhua, Lu Haijun
Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao, China.
Peking University Cancer Hospital, Beijing, China.
Transl Oncol. 2024 Jul;45:101990. doi: 10.1016/j.tranon.2024.101990. Epub 2024 May 11.
With regard to head and neck squamous cell carcinoma (HNSCC), its occurrence and advancement are controlled by genetic and epigenetic anomalies. PIWI-interacting RNAs (piRNAs) are recognized with significance in tumor, but the precise molecular mechanisms of piRNAs in HNSCC largely remain undisclosed.
Differentially expressed piRNAs were identified by RNA sequencing. The expression of piR-hsa-23533 was evaluated using quantitative real-time PCR and RNA in situ hybridization. The impacts of piR-hsa-23533 on the proliferation and apoptosis of HNSCC cells were investigated by a series of in vitro and in vivo assays.
piR-hsa-23533 exhibits upregulation within HNSCC cells and tissues. Besides, piR-hsa-23533 overexpression promotes proliferation while inhibiting apoptosis in vitro and in vivo, while piR-hsa-23533 silencing has an opposite function. From the mechanistic perspective, piR-hsa-23533 can bind to Ubiquitin-specific protease 7 (USP7), as shown through RNA pull-down and RNA immunoprecipitation assays, promoting USP7 mRNA and protein expression.
These findings highlight the functional importance of piR-hsa-23533 in HNSCC and may assist in the development of anti-HNSCC therapeutic target.
关于头颈部鳞状细胞癌（HNSCC），其发生和进展受遗传和表观遗传异常控制。PIWI相互作用RNA（piRNA）在肿瘤中具有重要意义，但piRNA在HNSCC中的精确分子机制很大程度上仍未明确。
通过RNA测序鉴定差异表达的piRNA。使用定量实时PCR和RNA原位杂交评估piR-hsa-23533的表达。通过一系列体外和体内实验研究piR-hsa-23533对HNSCC细胞增殖和凋亡的影响。
piR-hsa-23533在HNSCC细胞和组织中呈上调表达。此外，piR-hsa-23533过表达在体外和体内均促进增殖并抑制凋亡，而piR-hsa-23533沉默则具有相反作用。从机制角度来看，通过RNA下拉和RNA免疫沉淀实验表明，piR-hsa-23533可与泛素特异性蛋白酶7（USP7）结合，促进USP7 mRNA和蛋白表达。
这些发现突出了piR-hsa-23533在HNSCC中的功能重要性，并可能有助于抗HNSCC治疗靶点的开发。
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