sp. nov., a novel member isolated from coastal mudflat sediment of Meishan Islandin the East China Sea.

A Gram-negative, rod-shaped, non-motile and strictly aerobic strain, designated NBU2979, was isolated from a coastal mudflat located on Meishan Island in the East China Sea. Strain NBU2979 grew optimally at 32 °C, with 2.0 % NaCl (w/v) and at pH 7.0-7.5. The predominant fatty acid (>10 %) was iso-C. The major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylcholine, an unidentified glycolipid, two unidentified aminophospholipids, an unidentified phospholipid and an unidentified lipid. The only respiratory quinone was ubiquinone-8. Comparative analysis of 16S rRNA gene sequences showed that strain NBU2979 exhibited highest similarity to F2 (98.0 %), S1101 (97.5 %), KMM 3900 (96.6 %), 3539 (95.5 %), sw153 (95.2 %) and S6413 (94.9 %). Phylogenetic analyses indicated that strain NBU2979 clustered with the genus and was closely related to strain F2. The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU2979 and related species of genus were well below the threshold limit for prokaryotic species delineation. The DNA G+C content of strain NBU2979 was 51.6 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU2979 (=KCTC 82911=MCCC 1K06402) is considered to be a representative of a novel species in the genus , for which the name sp. nov. is proposed.