College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
J Invertebr Pathol. 2024 Jul;205:108141. doi: 10.1016/j.jip.2024.108141. Epub 2024 May 22.
Electron-transferring flavoprotein (Etf) and its dehydrogenase (Etfdh) are integral components of the electron transport chain in mitochondria. In this study, we characterize two putative etf genes (Bbetfa and Bbetfb) and their dehydrogenase gene Bbetfdh in the entomopathogenic fungus Beauveria bassiana. Individual deletion of these genes caused a significant reduction in vegetative growth, conidiation, and delayed conidial germination. Lack of these genes also led to abnormal metabolism of fatty acid and increasing lipid body accumulation. Furthermore, the virulence of Bbetfs and Bbetfdh deletion mutants was severely impaired due to decreasing infection structure formation. Additionally, all deletion strains showed reduced ATP synthesis compared to the wild-type strain. Taken together, Bbetfa and Bbetfb, along with Bbetfdh, play principal roles in fungal vegetative growth, conidiation, conidial germination, and pathogenicity of B. bassiana due to their essential functions in fatty acid metabolism.
电子转移黄素蛋白（Etf）及其脱氢酶（Etfdh）是线粒体电子传递链的组成部分。在这项研究中，我们鉴定了昆虫病原真菌白僵菌中的两个假定的 etf 基因（Bbetfa 和 Bbetfb）及其脱氢酶基因 Bbetfdh。这些基因的单独缺失导致营养生长、产孢和分生孢子萌发显著减少。这些基因的缺失还导致脂肪酸代谢异常和脂滴积累增加。此外，由于感染结构形成减少，Bbetfs 和 Bbetfdh 缺失突变体的毒力严重受损。此外，与野生型菌株相比，所有缺失菌株的 ATP 合成均减少。综上所述，Bbetfa 和 Bbetfb 以及 Bbetfdh 由于在脂肪酸代谢中具有重要功能，因此在白僵菌的营养生长、产孢、分生孢子萌发和致病性中发挥主要作用。
