Department of Chemistry-Biology and Agriculture, Interdisciplinary Faculty of Biology Sciences and Health, University of Sonora, Navojoa C.P. 85880, Mexico.
Laboratory of Immunology and Virology, Falculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolás de los Garza C.P. 66455, Mexico.
Int J Mol Sci. 2024 May 18;25(10):5514. doi: 10.3390/ijms25105514.
Rotavirus is the main cause of acute diarrhea in children up to five years of age. In this regard, probiotics are commonly used to treat or prevent gastroenteritis including viral infections. The anti-rotavirus effect of and , by reducing viral infectivity and improving IFN-type I response, has been previously reported. The present study aimed to study the effect of and/or on modulating the antiviral cellular immune response mediated by IFN-γ, IL-10, SOCS3, STAT1, and STAT2 genes in rotavirus-infected cells. To determine the mRNA relative expression of these genes, HT-29 cells were treated with and alone or in combination, followed by rotavirus infection. In addition, infected cells were treated with and/or . Cellular RNA was purified, used for cDNA synthesis, and amplified by qPCR. Our results demonstrated that the combination of and stimulates the antiviral cellular immune response by upregulating IFN-γ and may block pro-inflammatory cytokines by upregulating IL-10 and SOCS3. The results of our study indicated that , , or their combination improve antiviral cellular immune response and might modulate pro-inflammatory responses.
轮状病毒是导致 5 岁以下儿童急性腹泻的主要原因。在这方面，益生菌通常用于治疗或预防包括病毒感染在内的胃肠炎。先前已经报道了 和 通过降低病毒感染力和改善 I 型干扰素反应来抗轮状病毒的作用。本研究旨在研究 和/或 对调节轮状病毒感染细胞中由 IFN-γ、IL-10、SOCS3、STAT1 和 STAT2 基因介导的抗病毒细胞免疫反应的影响。为了确定这些基因的 mRNA 相对表达，用 和 单独或联合处理 HT-29 细胞，然后进行轮状病毒感染。此外，用 和/或 处理感染细胞。纯化细胞 RNA，用于 cDNA 合成，并通过 qPCR 进行扩增。我们的结果表明， 和 的组合通过上调 IFN-γ 刺激抗病毒细胞免疫反应，并且可能通过上调 IL-10 和 SOCS3 来阻断促炎细胞因子。我们的研究结果表明， 、 或它们的组合可以改善抗病毒细胞免疫反应，并可能调节促炎反应。
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