循环中的 IL-35 调节性 B 细胞与甲状腺相关眼病有关。
The circulating IL-35 regulatory B cells are associated with thyroid associated opthalmopathy.
机构信息
Department of Ophthalmology, People's Hospital of Ningxia Hui Autonomous Region, The Third Affiliated Clinical College of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China.
Medical Science Research Institution of Ningxia Hui Autonomous Region, Medical Sci-Tech Research Center of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China.
出版信息
Immun Inflamm Dis. 2024 May;12(5):e1304. doi: 10.1002/iid3.1304.
BACKGROUND
Thyroid-associated ophthalmopathy (TAO) is the most common orbital disease in adults, potentially leading to disfigurement and visual impairment. However, the causes of TAO are not fully understood. IL-35B cells are a newly identified regulatory B cells (Bregs) in maintaining immune balance in various autoimmune diseases. Yet, the influence of IL-35Bregs in TAO remains unexplored.
METHODS
This study enrolled 36 healthy individuals and 14 TAO patients. We isolated peripheral blood mononuclear cells and stimulated them with IL-35 and CpG for 48 h. Flow cytometry was used to measure the percentages of IL-35Bregs.
RESULTS
The percentage of circulating IL-35Bregs was higher in TAO patients, and this increase correlated positively with disease activity. IL-35 significantly increased the generation of IL-35Bregs in healthy individuals. However, B cells from TAO patients exhibited potential impairment in transitioning into IL-35Breg phenotype under IL-35 stimulation.
CONCLUSIONS
Our results suggest a potential role of IL-35Bregs in the development of TAO, opening new avenues for understanding disease mechanisms and developing therapeutic approaches.
背景
甲状腺相关眼病（TAO）是成人中最常见的眼眶疾病，可能导致容貌受损和视力障碍。然而，TAO 的病因尚未完全明了。IL-35B 细胞是一种新发现的调节性 B 细胞（Bregs），在各种自身免疫性疾病中维持免疫平衡。然而，IL-35Bregs 在 TAO 中的影响仍未被探索。
方法
本研究纳入 36 名健康个体和 14 名 TAO 患者。我们分离外周血单核细胞，并用 IL-35 和 CpG 刺激 48 小时。流式细胞术用于测量 IL-35Bregs 的百分比。
结果
TAO 患者循环中 IL-35Bregs 的百分比较高，且这种增加与疾病活动度呈正相关。IL-35 可显著增加健康个体中 IL-35Bregs 的生成。然而，TAO 患者的 B 细胞在 IL-35 刺激下向 IL-35Breg 表型转化的能力存在潜在缺陷。
结论
我们的结果提示 IL-35Bregs 在 TAO 的发生发展中可能具有一定作用，为理解疾病机制和开发治疗方法开辟了新的途径。
Zotero 插件