State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Ministry of Agriculture, Beijing, China.
Mol Plant Pathol. 2024 Jun;25(6):e13459. doi: 10.1111/mpp.13459.
F-box protein is a subunit of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which plays a critical role in regulating different pathways in plant immunity. In this study, we identified the rice (Oryza sativa) F-box protein OsFBX156, which targets the heat shock protein 70 (OsHSP71.1) to regulate resistance to the rice blast fungus Magnaporthe oryzae. Overexpression of OsFBX156 or knockout of OsHSP71.1 in rice resulted in the elevation of pathogenesis-related (PR) genes and an induction burst of reactive oxygen species (ROS) after flg22 and chitin treatments, thereby enhancing resistance to M. oryzae. Furthermore, OsFBX156 can promote the degradation of OsHSP71.1 through the 26S proteasome pathway. This study sheds lights on a novel mechanism wherein the F-box protein OsFBX156 targets OsHSP71.1 for degradation to promote ROS production and PR gene expression, thereby positively regulating rice innate immunity.
F-box 蛋白是 SCF（SKP1-CUL1-F-box 蛋白）E3 泛素连接酶复合物的一个亚基，在植物免疫的不同途径中发挥着关键作用。在这项研究中，我们鉴定了水稻（Oryza sativa）F-box 蛋白 OsFBX156，它靶向热休克蛋白 70（OsHSP71.1）以调节对稻瘟病菌（Magnaporthe oryzae）的抗性。在水稻中过表达 OsFBX156 或敲除 OsHSP71.1 会导致病程相关（PR）基因的上调和 flg22 和几丁质处理后活性氧（ROS）的诱导爆发，从而增强对 M. oryzae 的抗性。此外，OsFBX156 可以通过 26S 蛋白酶体途径促进 OsHSP71.1 的降解。这项研究揭示了一种新的机制，即 F-box 蛋白 OsFBX156 靶向 OsHSP71.1 进行降解，以促进 ROS 产生和 PR 基因表达，从而正向调节水稻先天免疫。
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