School of Pharmaceutical Science, Nanchang University, Nanchang 330006, Jiangxi, China.
Molecular Physiology Division, Faculty of Science, Beni-Suef University, Egypt.
Ultrason Sonochem. 2024 Jul;107:106923. doi: 10.1016/j.ultsonch.2024.106923. Epub 2024 May 25.
The utilization of metallic nanoparticles in bio-nanofabrication holds significant potential in the field of applied research. The current study applied and compared integrated ultrasonic-microwave-assisted extraction (US/MICE), ultrasonic extraction (USE), microwave-assisted extraction (MICE), and maceration (MAE) to extract total phenolic content (TPC). In addition, the study examined the antioxidant activity of Commiphora gileadensis (Cg) leaf. The results demonstrated that the TPC of US/MICE exhibited the maximum value at 59.34 ± 0.007 mg GAE/g DM. Furthermore, at a concentration of 10 μg/mL, TPC displayed a significant scavenging effect on DPPH (56.69 %), with an EC (6.48 μg/mL). Comprehensive metabolite profiling of the extract using UPLC-qTOF-MS/MS was performed to identify active agents. A total of 64 chromatographic peaks were found, out of which 60 were annotated. The most prevalent classes of metabolites found were polyphenols (including flavonoids and lignans), organic compounds and their derivatives, amides and amines, terpenes, and fatty acid derivatives. Transmission electron microscopy (TEM) revealed the aggregate size of the synthesized nanoparticles and the spherical shape of C. gileadensis-mediated silver nanoparticles (Cg-AgNPs). The nanoparticles had a particle size ranging from 7.7 to 42.9 nm. The Cg-AgNPs exhibited more inhibition zones against S. aureus and E. coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Cg-extract, AgNPs, and Cg-AgNPs were also tested. This study demonstrated the feasibility of using combined ultrasonic-microwave-assisted extraction to separate and extract chemicals from C. gileadensis on a large scale. These compounds have potential use in the pharmaceutical industry. Combining antibacterial and biocompatible properties in materials is vital for designing new materials for biomedical applications. Additionally, the results showed that the biocompatibility of the Ag-NPs using C. gileadensis extracts demonstrated outstanding antibacterial properties.
在应用研究领域，金属纳米粒子在生物纳米制造中的应用具有重要潜力。本研究应用并比较了集成超声-微波辅助提取（US/MICE）、超声提取（USE）、微波辅助提取（MICE）和浸渍提取（MAE）来提取总酚含量（TPC）。此外，该研究还考察了 Commiphora gileadensis（Cg）叶的抗氧化活性。结果表明，US/MICE 的 TPC 值在 59.34±0.007 mg GAE/g DM 时达到最大值。此外，在 10μg/mL 浓度下，TPC 对 DPPH（56.69%）具有显著的清除作用，EC（6.48μg/mL）。使用 UPLC-qTOF-MS/MS 对提取物进行综合代谢物谱分析，以鉴定活性物质。共发现 64 个色谱峰，其中 60 个被注释。发现的最常见代谢物类别是多酚（包括类黄酮和木脂素）、有机化合物及其衍生物、酰胺和胺、萜类化合物和脂肪酸衍生物。透射电子显微镜（TEM）显示了合成纳米粒子的聚集大小和 C. gileadensis 介导的银纳米粒子（Cg-AgNPs）的球形。纳米粒子的粒径范围为 7.7 至 42.9nm。Cg-AgNPs 对金黄色葡萄球菌和大肠杆菌的抑制圈更大。还测试了 Cg 提取物、AgNPs 和 Cg-AgNPs 的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。本研究证明了使用联合超声-微波辅助提取从 C. gileadensis 中分离和提取化学物质的可行性，可大规模进行。这些化合物在制药工业中有潜在的用途。在材料中结合抗菌和生物相容性特性对于设计用于生物医学应用的新材料至关重要。此外，结果表明，使用 C. gileadensis 提取物的 Ag-NPs 的生物相容性表现出出色的抗菌性能。
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