Plasma EBV quantification is associated with the efficacy of immune checkpoint blockade and disease monitoring in patients with primary pulmonary lymphoepithelioma-like carcinoma.

OBJECTIVES

Primary pulmonary lymphoepithelioma-like carcinoma (PLELC) is a subtype of lung carcinoma associated with the Epstein-Barr virus (EBV). The clinical predictive biomarkers of immune checkpoint blockade (ICB) in PLELC require further investigation.

METHODS

We prospectively analysed EBV levels in the blood and immune tumor biomarkers of 31 patients with ICB-treated PLELC. Viral and DNA fragments in the plasma were quantified in parallel using quantitative polymerase chain reaction.

RESULTS

Progression-free survival (PFS) was significantly longer in high or high groups. A longer PFS was also observed in patients with both high plasma or and PD-L1 ≥ 1%. Intriguingly, the tumor mutational burden was inversely correlated with and . Plasma EBV load was negatively associated with intratumoral CD8 immune cell infiltration. Dynamic changes in plasma EBV DNA level were in accordance with the changes in tumor volume. An increase in EBV DNA levels during treatment indicated molecular progression that preceded the imaging progression by several months.

CONCLUSIONS

Plasma EBV DNA could be a useful and easy-to-use biomarker for predicting the clinical activity of ICB in PLELC and could serve to monitor disease progression earlier than computed tomography imaging.