Key Lab of Organic-Based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
Plant-Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Padualaan 8, Utrecht, 3584 CH, the Netherlands.
Microbiome. 2024 Jun 5;12(1):101. doi: 10.1186/s40168-024-01814-z.
Plant microbiota contributes to plant growth and health, including enhancing plant resistance to various diseases. Despite remarkable progress in understanding diseases resistance in plants, the precise role of rhizosphere microbiota in enhancing watermelon resistance against soil-borne diseases remains unclear. Here, we constructed a synthetic community (SynCom) of 16 core bacterial strains obtained from the rhizosphere of grafted watermelon plants. We further simplified SynCom and investigated the role of bacteria with synergistic interactions in promoting plant growth through a simple synthetic community.
Our results demonstrated that the SynCom significantly enhanced the growth and disease resistance of ungrafted watermelon grown in non-sterile soil. Furthermore, analysis of the amplicon and metagenome data revealed the pivotal role of Pseudomonas in enhancing plant health, as evidenced by a significant increase in the relative abundance and biofilm-forming pathways of Pseudomonas post-SynCom inoculation. Based on in vitro co-culture experiments and bacterial metabolomic analysis, we selected Pseudomonas along with seven other members of the SynCom that exhibited synergistic effects with Pseudomonas. It enabled us to further refine the initially constructed SynCom into a simplified SynCom comprising the eight selected bacterial species. Notably, the plant-promoting effects of simplified SynCom were similar to those of the initial SynCom. Furthermore, the simplified SynCom protected plants through synergistic effects of bacteria.
Our findings suggest that the SynCom proliferate in the rhizosphere and mitigate soil-borne diseases through microbial synergistic interactions, highlighting the potential of synergistic effects between microorganisms in enhancing plant health. This study provides a novel insight into using the functional SynCom as a promising solution for sustainable agriculture. Video Abstract.
植物微生物群有助于植物生长和健康，包括增强植物对各种疾病的抵抗力。尽管人们在理解植物的抗病性方面取得了显著进展，但根际微生物群在增强西瓜对土传病害的抵抗力方面的确切作用仍不清楚。在这里，我们构建了一个由嫁接西瓜植物根际中获得的 16 个核心细菌菌株组成的合成群落（SynCom）。我们进一步简化了 SynCom，并通过一个简单的合成群落研究了具有协同相互作用的细菌在促进植物生长中的作用。
我们的结果表明，SynCom 显著增强了在非无菌土壤中生长的未嫁接西瓜的生长和抗病性。此外，扩增子和宏基因组数据分析表明，假单胞菌在增强植物健康方面起着关键作用，这表现在接种 SynCom 后假单胞菌的相对丰度和生物膜形成途径显著增加。基于体外共培养实验和细菌代谢组学分析，我们选择了假单胞菌以及 SynCom 中另外七种与假单胞菌表现出协同作用的成员。这使我们能够进一步将最初构建的 SynCom 简化为一个包含 8 种选定细菌的简化 SynCom。值得注意的是，简化 SynCom 的植物促进作用与初始 SynCom 相似。此外，简化 SynCom 通过细菌的协同作用保护植物。
我们的研究结果表明，SynCom 在根际中增殖，并通过微生物协同相互作用减轻土传病害，突出了微生物之间协同作用增强植物健康的潜力。本研究为利用功能 SynCom 作为可持续农业的有前景的解决方案提供了新的见解。视频摘要。
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