Three new antinociceptive diterpenoids from the fruits of .

Investigation of the fruits of G. Don led to the isolation of three new grayanane-type diterpenoids, rhodomolleins LIV-LVI (). The structures and absolute configurations of new compounds were fully elucidated by spectroscopic analysis and single-crystal X-ray diffraction, including HRESIMS, 1 D and 2 D NMR data. Compounds - were evaluated for analgesic activities utilizing an acetic acid-induced writhing test in mice. Compound showed a significant antinociceptive effect with writhe inhibition rates of 72.9% and 100% at doses of 6 mg/kg and 20 mg/kg in mice, respectively. The binding mode of to -ethylmaleimide-sensitive factor (NSF, PDB: 6IP2) was explored by molecular docking, indicating the presence of hydrogen bond interactions which account for its analgesic activity.