Analysis of the anti-PCV2 mechanism of based on non-target metabolomics and high-throughput molecular docking.

Our previous studies have revealed that possesses inhibitory effects on PCV2 proliferation , although the underlying mechanisms remain elusive. Probiotics like are known to exert antiviral through their metabolites. Therefore, in this study, non-targeted metabolomics was used to detect the changes in metabolites of after 24 h of proliferation. Subsequently, high-throughput molecular docking was utilized to analyze the docking scores of these metabolites with PCV2 Cap and Rep, aiming to identify compounds with potential anti-PCV2 effects. The results demonstrated that 128 compounds such as Dl-lactate were significantly increased. The results of high-throughput molecular docking indicated that compounds such as ergocristine, and telmisartan formed complexes with Cap and Rep, suggesting their potential anti-PCV2 properties. Furthermore, compounds like vitamin C, exhibit pharmacological effects consistent with adding credence to the idea that may exert pharmacological effects through its metabolites. These results will provide a foundation for the study of .