藤黄果提取物漱口液作为口腔卫生方案辅助剂的生物学特性的构建和评估：体外分析。
Formulation and assessment of biological properties of garcinia indica fruit extract mouthrinse as an adjunct to oral hygiene regimen: an in vitro analysis.
机构信息
Manipal Academy of Higher Education, Manipal College of Dental Sciences, Department of Periodontology, Manipal, Karnataka, India.
Manipal Academy of Higher Education, Manipal College of Pharmaceutical Sciences, Department of Pharmaceutics, Manipal, Karnataka, India.
出版信息
J Appl Oral Sci. 2024 Jun 10;32:e20230291. doi: 10.1590/1678-7757-2023-0291. eCollection 2024.
UNLABELLED
The prevalence of gingivitis is substantial within the general population, necessitating rigorous oral hygiene maintenance.
OBJECTIVE
This study assessed a Garcinia indica (GI) fruit extract-based mouthrinse, comparing it to a 0.1% turmeric mouthrinse and a 0.2% Chlorhexidine (CHX) mouthrinse. The evaluation encompassed substantivity, staining potential, antimicrobial efficacy and cytocompatibility.
METHODOLOGY
The study employed 182 tooth sections. For antimicrobial analysis, 64 extracted human teeth coated with a polymicrobial biofilm were divided into four groups, each receiving an experimental mouthrinse or serving as a control group with distilled water. Microbial reduction was assessed through colony forming units (CFU). Substantivity was evaluated on 54 human tooth sections using a UV spectrophotometer, while staining potential was examined on 64 tooth sections. Cytocompatibility was tested using colorimetric assay to determine non-toxic levels of 0.2% GI fruit extract, 0.1% Turmeric, and 0.2% CHX.
RESULTS
Data were analysed with one-way ANOVA (α=0.05). Cell viability was highly significant (p<0.001) in the 0.2% GI group (64.1±0.29) compared to 0.1% Turmeric (40.2±0.34) and 0.2% CHX (10.95±1.40). For antimicrobial activity, both 0.2% GI (20.18±4.81) and 0.2% CHX (28.22±5.41) exhibited no significant difference (P>0.05) at end of 12 hours. However, 0.1% Turmeric showed minimal CFU reduction (P<0.001). Substantivity results at 360 minutes indicated statistically significant higher mean release rate in 0.1%Turmeric (12.47±5.84 ) when compared to 0.2% GI (5.02±3.04) and 0.2% CHX (4.13±2.25) (p<0.001). The overall discoloration changes (∆E) were more prominent in the 0.2% CHX group (18.65±8.3) compared to 0.2% GI (7.61±2.4) and 0.1% Turmeric (7.32±4.9) (P<0.001).
CONCLUSION
This study supports 0.2% GI and 0.1% Turmeric mouth rinses as potential natural alternatives to chemical mouth rinses. These findings highlight viability of these natural supplements in oral healthcare.
未加标签
牙龈炎在普通人群中普遍存在，需要严格保持口腔卫生。
目的
本研究评估了一种基于藤黄果（GI）提取物的漱口液，将其与 0.1%姜黄漱口液和 0.2%洗必泰（CHX）漱口液进行比较。评估包括持续性、染色潜力、抗菌功效和细胞相容性。
方法
本研究使用了 182 个牙段。对于抗菌分析，将 64 个涂有微生物生物膜的人牙分为四组，每组分别接受实验性漱口液或用蒸馏水作为对照组。通过菌落形成单位（CFU）评估微生物减少。在 54 个人牙段上使用紫外分光光度计评估持续性，在 64 个人牙段上评估染色潜力。使用比色法测定细胞毒性试验以确定 0.2%GI 果提取物、0.1%姜黄和 0.2%CHX 的无毒水平。
结果
使用单向方差分析（α=0.05）进行数据分析。0.2%GI 组（64.1±0.29）的细胞活力显著（p<0.001）高于 0.1%姜黄组（40.2±0.34）和 0.2%CHX 组（10.95±1.40）。对于抗菌活性，在 12 小时结束时，0.2%GI（20.18±4.81）和 0.2%CHX（28.22±5.41）均无显著差异（P>0.05）。然而，0.1%姜黄显示最小的 CFU 减少（P<0.001）。在 360 分钟时的持续性结果表明，与 0.2%GI（5.02±3.04）和 0.2%CHX（4.13±2.25）相比，0.1%姜黄的平均释放率具有统计学意义上的更高（12.47±5.84）（p<0.001）。总体变色变化（∆E）在 0.2%CHX 组中更为明显（18.65±8.3），与 0.2%GI（7.61±2.4）和 0.1%姜黄（7.32±4.9）相比（P<0.001）。
结论
本研究支持 0.2%GI 和 0.1%姜黄漱口液作为化学漱口液的潜在天然替代品。这些发现突出了这些天然补充剂在口腔保健中的活力。
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