拟南芥隐花色素与 SOG1 相互作用，促进 DNA 双链断裂的修复。
Arabidopsis cryptochromes interact with SOG1 to promote the repair of DNA double-strand breaks.
机构信息
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China; Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
出版信息
Biochem Biophys Res Commun. 2024 Sep 10;724:150233. doi: 10.1016/j.bbrc.2024.150233. Epub 2024 Jun 7.
Cryptochromes (CRYs) are blue light (BL) photoreceptors to regulate a variety of physiological processes including DNA double-strand break (DSB) repair. SUPPRESSOR OF GAMMA RADIATION 1 (SOG1) acts as the central transcription factor of DNA damage response (DDR) to induce the transcription of downstream genes, including DSB repair-related genes BRCA1 and RAD51. Whether CRYs regulate DSB repair by directly modulating SOG1 is unknown. Here, we demonstrate that CRYs physically interact with SOG1. Disruption of CRYs and SOG1 leads to increased sensitivity to DSBs and reduced DSB repair-related genes' expression under BL. Moreover, we found that CRY1 enhances SOG1's transcription activation of DSB repair-related gene BRCA1. These results suggest that the mechanism by which CRYs promote DSB repair involves positive regulation of SOG1's transcription of its target genes, which is likely mediated by CRYs-SOG1 interaction.
隐花色素（CRYs）是蓝光（BL）感受器，可调节多种生理过程，包括 DNA 双链断裂（DSB）修复。GAMMA 辐射抑制因子 1（SOG1）作为 DNA 损伤反应（DDR）的核心转录因子，诱导下游基因，包括 DSB 修复相关基因 BRCA1 和 RAD51 的转录。CRYs 是否通过直接调节 SOG1 来调节 DSB 修复尚不清楚。在这里，我们证明 CRYs 与 SOG1 发生物理相互作用。在 BL 下，破坏 CRYs 和 SOG1 会导致对 DSB 的敏感性增加和 DSB 修复相关基因表达减少。此外，我们发现 CRY1 增强了 SOG1 对 DSB 修复相关基因 BRCA1 的转录激活。这些结果表明，CRYs 促进 DSB 修复的机制涉及对 SOG1 靶基因转录的正向调节，这可能是由 CRYs-SOG1 相互作用介导的。
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