School of Agriculture, Ludong University, Yantai 264025, China.
Yantai Growth Drivers Conversion Research Institute and Yantai Science and Technology Achievement Transfer and Transformation Demonstration Base, Yantai 264001, China.
Int J Mol Sci. 2024 May 23;25(11):5677. doi: 10.3390/ijms25115677.
Mycoviruses are usually transmitted horizontally via hyphal anastomosis and vertically through sporulation in natural settings. Oyster mushroom spherical virus (OMSV) is a mycovirus that infects , with horizontal transmission via hyphal anastomosis. However, whether OMSV can be vertically transmitted is unclear. This study aimed to investigate the transmission characteristics of OMSV to progeny via basidiospores and horizontally to a new host. A total of 37 single-basidiospore offspring were obtained from OMSV-infected and for Western blot detection of OMSV. The OMSV-carrying rate among monokaryotic isolates was 19% in and 44% in . Then, OMSV-free and OMSV-infected monokaryotic isolates were selected for hybridization with harvested dikaryotic progeny strains. Western blot analyses of the offspring revealed that the OMSV transmission efficiency was 50% in and 75% in , indicating vertical transmission via sexual basidiospores. Furthermore, we observed the horizontal transfer of OMSV from to . OMSV infection in resulted in significant inhibition of mycelial growth and yield loss. This study was novel in reporting the vertical transmission of OMSV through basidiospores, and its infection and pathogenicity in a new host .
真菌病毒通常通过菌丝融合在自然环境中水平传播，通过孢子在垂直方向传播。牡蛎菇球形病毒（OMSV）是一种感染的真菌病毒，通过菌丝融合进行水平传播。然而，OMSV 是否可以垂直传播尚不清楚。本研究旨在调查 OMSV 通过担孢子垂直传播给后代和水平传播给新宿主的特性。从 OMSV 感染的和中获得了 37 个单担孢子后代，用于 Western blot 检测 OMSV。在和中单核分离物中携带 OMSV 的比率分别为 19%和 44%。然后，选择无 OMSV 和携带 OMSV 的单核分离物与收获的双核后代菌株进行杂交。对后代的 Western blot 分析表明，在和中，OMSV 的传播效率分别为 50%和 75%，表明通过有性担孢子垂直传播。此外，我们观察到 OMSV 从向的水平转移。在中，OMSV 感染导致菌丝生长显著抑制和产量损失。本研究的新颖之处在于报道了 OMSV 通过担孢子的垂直传播，以及其在新宿主中的感染和致病性。
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