Department of Medical Neuroscience, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Key University Laboratory of Metabolism and Health of Guangdong, Southern University of Science and Technology, Shenzhen 518055, China.
Int J Mol Sci. 2024 May 23;25(11):5702. doi: 10.3390/ijms25115702.
Glioblastoma (GBM) is the most common malignant brain tumor in adults. Despite advancements in treatment, the prognosis for patients with GBM remains poor due to its aggressive nature and resistance to therapy. CRISPR-based genetic screening has emerged as a powerful tool for identifying genes crucial for tumor progression and treatment resistance, offering promising targets for tumor therapy. In this review, we provide an overview of the recent advancements in CRISPR-based genetic screening approaches and their applications in GBM. We highlight how these approaches have been used to uncover the genetic determinants of GBM progression and responsiveness to various therapies. Furthermore, we discuss the ongoing challenges and future directions of CRISPR-based screening methods in advancing GBM research.
胶质母细胞瘤（GBM）是成人中最常见的恶性脑肿瘤。尽管治疗取得了进展，但由于其侵袭性和对治疗的耐药性，GBM 患者的预后仍然不佳。基于 CRISPR 的基因筛选已成为鉴定肿瘤进展和治疗耐药性关键基因的强大工具，为肿瘤治疗提供了有希望的靶点。在这篇综述中，我们提供了基于 CRISPR 的基因筛选方法的最新进展及其在 GBM 中的应用概述。我们强调了这些方法如何用于揭示 GBM 进展和对各种治疗方法的反应的遗传决定因素。此外，我们讨论了基于 CRISPR 的筛选方法在推进 GBM 研究方面的持续挑战和未来方向。
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