全基因组鉴定基因家族及其对非生物胁迫响应的功能分析

Genome-Wide Identification of Gene Family in and Functional Analysis of in Response to Abiotic Stress.

机构信息

College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China.

College of Plant Science, Jilin University, Changchun 130062, China.

出版信息

Int J Mol Sci. 2024 Jun 4;25(11):6173. doi: 10.3390/ijms25116173.

DOI:10.3390/ijms25116173

PMID:38892361

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11173228/

Abstract

has important uses in medicine, wind breaking, and sand fixation. The CHY-zinc-finger and RING-finger (CHYR) proteins are crucial for plant growth, development, and environmental adaptation; however, genetic data regarding the family remain scarce. We aimed to investigate the gene family in and its response to abiotic stress, and identified 18 new genes from whole-genome data, categorized into 3 subclasses through a phylogenetic analysis. Gene structure, protein domains, and conserved motifs analyses revealed an exon-intron structure and conserved domain similarities. A chromosome localization analysis showed distribution across 12 chromosomes. A promoter analysis revealed abiotic stress-, light-, and hormone-responsive elements. An RNA-sequencing expression pattern analysis revealed positive responses of genes to salt, alkali, and drought stress. overexpression considerably enhanced alkali and drought tolerance in . These findings shed light on SaCHYR's function and the resistance mechanisms of , presenting new genetic resources for crop resistance breeding.

摘要

在医学、防风固沙方面具有重要用途。CHY-锌指和 RING-指（CHYR）蛋白对植物的生长、发育和环境适应至关重要；然而，关于该家族的遗传数据仍然很少。我们旨在研究及其对非生物胁迫的响应，并从全基因组数据中鉴定出 18 个新的基因，通过系统发育分析将其分为 3 个亚类。基因结构、蛋白结构域和保守基序分析揭示了外显子-内含子结构和保守结构域的相似性。染色体定位分析表明其分布于 12 条染色体上。启动子分析表明存在非生物胁迫、光照和激素响应元件。RNA-seq 表达模式分析表明，基因对盐、碱和干旱胁迫有正响应。在中过表达基因可显著提高耐碱和耐旱性。这些发现揭示了 SaCHYR 的功能和抵抗机制，为作物抗性育种提供了新的遗传资源。