Department of Food Science and Technology, College of Science and Convergence Technology, Seoul Women's University, 621 Hwarang-ro, Nowon-gu, Seoul 01797, Republic of Korea.
Int J Mol Sci. 2024 Jun 6;25(11):6278. doi: 10.3390/ijms25116278.
The biological activities and related mechanisms of curcumin, a major polyphenolic compound in turmeric, the rhizome of , have been extensively investigated. Due to its poor solubility in water, the analysis of curcumin's biological activities is limited in most aqueous experimental systems. In the present study, the effects of polyvinyl alcohol (PVA), a dietary-compatible vehicle, on the solubility, stability, cellular uptake, and bioactivities of curcumin were investigated. Curcumin solubility was improved significantly by PVA; the color intensity of curcumin aqueous solution in the presence of PVA increased concentration-dependently with its peak shift to a shorter wavelength. Improved suspension stability and photostability of curcumin in an aqueous solution were also observed in the presence of PVA, even at 62.5 μg/mL. The scavenging activities of curcumin against DPPH, ABTS, AAPH radicals, and nitric oxide were enhanced significantly in the presence of PVA. PVA at 250 μg/mL also significantly enhanced the cytotoxic activity of curcumin against both HCT 116 colon cancer and INT 407 (HeLa-derived) embryonic intestinal cells by reducing the IC from 16 to 11 μM and 25 to 15 μM, respectively. PVA improved the cellular uptake of curcumin in a concentration-dependent manner in INT 407 cells; it increased the cellular levels more effectively at lower curcumin treatment concentrations. The present results indicate that PVA improves the solubility and stability of curcumin, and changes in these chemical behaviors of curcumin in aqueous systems by PVA could enhance the bioavailability and pharmacological efficacy of curcumin.
姜黄，是姜科植物姜黄的根茎，其中的主要多酚化合物姜黄素，其生物活性和相关机制已得到广泛研究。由于其在水中的溶解度较差，因此在大多数水性实验系统中，姜黄素的生物活性分析受到限制。本研究考察了聚乙烯醇（PVA）作为一种可食用载体对姜黄素的溶解度、稳定性、细胞摄取和生物活性的影响。PVA 显著提高了姜黄素的溶解度；PVA 存在时，姜黄素水溶液的颜色强度随浓度增加呈浓度依赖性增加，其峰值向短波长移动。即使在 62.5μg/mL 时，也观察到 PVA 存在时姜黄素在水性溶液中的悬浮稳定性和光稳定性得到改善。PVA 显著增强了姜黄素对 DPPH、ABTS、AAPH 自由基和一氧化氮的清除活性。250μg/mL 的 PVA 还通过将姜黄素对 HCT 116 结肠癌细胞和 INT 407（HeLa 衍生）胚胎肠细胞的 IC 分别从 16μM 降低至 11μM 和从 25μM 降低至 15μM，显著增强了姜黄素的细胞毒性活性。PVA 以浓度依赖的方式增强了 INT 407 细胞中姜黄素的细胞摄取；在较低的姜黄素处理浓度下，它更有效地增加了细胞内水平。这些结果表明，PVA 提高了姜黄素的溶解度和稳定性，PVA 改变了姜黄素在水性系统中的这些化学行为，从而提高了姜黄素的生物利用度和药理功效。
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