前列环素在离体人脐动脉中的形成与作用

Formation and action of prostacyclin in the isolated human umbilical artery.

作者信息

Hamberg M, Tuvemo T, Svensson J, Jonsson C E

出版信息

Acta Physiol Scand. 1979 Jul;106(3):289-92. doi: 10.1111/j.1748-1716.1979.tb06400.x.

DOI:10.1111/j.1748-1716.1979.tb06400.x

Abstract

The transformation of [1-14C]arachidonic acid by homogenates of human umbilical arteries was studied. The major compound formed was the stable end product of PGI2, i.e. 6-keto-PGF1 alpha (lactol form) as analyzed by gas-liquid chromatography-mass spectrometry. PGI2 was generated by incubating PGH2 with a lyophilized pig aorta microsome preparation. PGI2 concentrations around 10 ng/ml relaxed the human umbilical artery preparation significantly. Formation of PGI2 by umbilical arteries during pregnancy might be a mechanism for regulation of blood flow to the fetus.

摘要

研究了人脐动脉匀浆对[1-14C]花生四烯酸的转化。通过气液色谱-质谱分析，形成的主要化合物是前列环素（PGI2）的稳定终产物，即6-酮-前列腺素F1α（内酯形式）。通过将前列腺素H2（PGH2）与冻干的猪主动脉微粒体制剂一起孵育来生成PGI2。浓度约为10 ng/ml的PGI2可显著舒张人脐动脉制剂。妊娠期间脐动脉生成PGI2可能是调节胎儿血流的一种机制。