Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong 637009, PR China.
Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong 637009, PR China.
Sci Total Environ. 2024 Oct 1;945:174163. doi: 10.1016/j.scitotenv.2024.174163. Epub 2024 Jun 19.
A novel dual-signal fluorometric and colorimetric probe FMDH (5-FAM-Met-Asp-His-NH), incorporating a tripeptide (Met-Asp-His-NH) linked to 5-carboxyfluorescein (5-FAM), was firstly synthesised. FMDH demonstrated exceptional selectivity and sensitivity, rapid response, wide pH response range and robust anti-interference capabilities for monitoring Cu. This was achieved through a distinctive naked-eye colorimetric and fluorescent quenching behaviour. A good linearity within the range of 0-3 μM (R = 0.9914) was attained, and the limit of detection (LOD) for Cu was 47.4 nM. Furthermore, the FMDH-Cu ensemble responded to glyphosate with notable selectivity and sensitivity. A good linear correlation (R = 0.9926) was observed at the lower concentration range (2.4-7.8 μM) and achieving a detection limit as low as 29.9 nM. The response time of FMDH with Cu and glyphosate were less than 20 s, and the pH range of 7-11 that was suitable for practical application under physiological pH conditions. MTT assays confirmed that FMDH offers good permeability and low toxicity, facilitating successful application in imaging analysis of Cu and glyphosate in living cells and zebrafish. In addition, FMDH was employed in the detection of these analytes in real water samples. Cost-effective, highly sensitive and easily prepared FMDH-impregnated test strips were developed for the efficient visual detection of Cu and glyphosate under 365 nm UV light. Increasing concentrations of Cu and glyphosate resulted in notable colour changes under 365 nm UV light, enabling visual semi-quantitative analysis via a smartphone colour-analysis App.
一种新型的双信号荧光和比色探针 FMDH（5-FAM-Met-Asp-His-NH），由连接到 5-羧基荧光素（5-FAM）的三肽（Met-Asp-His-NH）组成，首次被合成。FMDH 对监测 Cu 表现出优异的选择性和灵敏度、快速响应、宽 pH 响应范围和强大的抗干扰能力。这是通过独特的肉眼比色和荧光猝灭行为实现的。在 0-3 μM 的范围内实现了良好的线性关系（R = 0.9914），Cu 的检测限（LOD）为 47.4 nM。此外，FMDH-Cu 配合物对草甘膦表现出显著的选择性和灵敏度。在较低的浓度范围（2.4-7.8 μM）观察到良好的线性相关性（R = 0.9926），并实现了低至 29.9 nM 的检测限。FMDH 与 Cu 和草甘膦的响应时间均小于 20 s，且在生理 pH 条件下适合实际应用的 pH 范围为 7-11。MTT 测定证实 FMDH 具有良好的通透性和低毒性，有助于成功应用于 Cu 和草甘膦在活细胞和斑马鱼中的成像分析。此外，FMDH 还用于检测实际水样中的这些分析物。开发了经济高效、高灵敏度且易于制备的 FMDH 浸渍测试条，用于在 365nm 紫外光下对 Cu 和草甘膦进行高效目视检测。随着 Cu 和草甘膦浓度的增加，在 365nm 紫外光下会发生明显的颜色变化，通过智能手机颜色分析应用程序可进行目视半定量分析。
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