Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs, Taiyuan, 030031, China.
BGI Genomics, Shenzhen, 518083, China.
Sci Data. 2024 Jun 21;11(1):657. doi: 10.1038/s41597-024-03489-5.
Broomcorn millet (Panicum miliaceum L.), known for its traits of drought resistance, adaptability to poor soil, short growth period, and high photosynthetic efficiency as a C plant, represents one of the earliest domesticated crops globally. This study reports the telomere-to-telomere (T2T) gap-free reference genome for broomcorn millet (AJ8) using PacBio high-fidelity (HiFi) long reads, Oxford Nanopore long-read technologies and high-throughput chromosome conformation capture (Hi-C) sequencing data. The size of AJ8 genome was approximately 834.7 Mb, anchored onto 18 pseudo-chromosomes. Notably, 18 centromeres and 36 telomeres were obtained. The assembled genome showed high quality in terms of completeness (BUSCO score: 99.6%, QV: 61.7, LAI value: 20.4). In addition, 63,678 protein-coding genes and 433.8 Mb (~52.0%) repetitive sequences were identified. The complete reference genome for broomcorn millet provides a valuable resource for genetic studies and breeding of this important cereal crop.
黍（Panicum miliaceum L.）以耐旱、适应贫瘠土壤、生长周期短和作为 C 植物的高光效等特性而闻名，是全球最早驯化的作物之一。本研究利用 PacBio 高保真（HiFi）长读长、Oxford Nanopore 长读长技术和高通量染色体构象捕获（Hi-C）测序数据，报道了黍（AJ8）的端粒到端粒（T2T）无间隙参考基因组。AJ8 基因组大小约为 834.7Mb，锚定在 18 个假染色体上。值得注意的是，获得了 18 个着丝粒和 36 个端粒。组装的基因组在完整性方面具有很高的质量（BUSCO 评分：99.6%，QV：61.7，LAI 值：20.4）。此外，鉴定出 63678 个蛋白质编码基因和 433.8Mb（~52.0%）重复序列。黍的完整参考基因组为该重要谷物作物的遗传研究和育种提供了宝贵的资源。
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