Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Federal University of Maranhão, São Luís, Maranhão, Brazil.
Bioprocess Biosyst Eng. 2024 Oct;47(10):1633-1645. doi: 10.1007/s00449-024-03055-8. Epub 2024 Jul 6.
This work aimed to define strategies to increase the bioproduction of 6 pentyl-α-pyrone (bioaroma). As first strategy, fermentations were carried out in the solid state, with agro-industrial residues: Mauritia flexuosa Liliopsida. and Manihot esculenta Crantz in isolation, conducting them with different nutrient solutions having Trichoderma harzianum as a fermenting fungus. Physicochemical characterizations, centesimal composition, lignocellulosic and mineral content and antimicrobial activity were required. Fermentations were conducted under different humidification conditions (water, nutrient solution without additives and nutrient solutions with glucose or sucrose) for 9 days. Bioaroma was quantified by gas chromatography, assisted by solid-phase microextraction. The results showed the low production of this compound in fermentations conducted with sweet cassava (around 6 ppm (w/w)). The low bioproduction with sweet cassava residues can probably be related to its starch-rich composition, homogeneous substrate, and low concentration of nutrients. Already using buriti, the absence of aroma production was detected. Probably the presence of silicon and high lignin content in buriti minimized the fungal activity, making it difficult to obtain the aroma of interest. Given the characteristics presented by the waste, a new strategy was chosen: mixing waste in a 1:1 ratio. This fermentation resulted in the production of 156.24 ppm (w/w) of aroma using the nutrient solution added with glucose. This combination, therefore, promoted more favorable environment for the process, possibly due to the presence of fermentable sugars from sweet cassava and fatty acids from the buriti peel, thus proving the possibility of an increase of around 2500% in the bioproduction of coconut aroma.
这项工作旨在定义提高 6-戊基-α-吡喃酮(生物香气)生物产量的策略。作为第一个策略,在固态发酵中使用农业工业残余物:Mauritia flexuosa Liliopsida 和 Manihot esculenta Crantz 进行发酵,用不同的营养溶液进行发酵,其中 Trichoderma harzianum 作为发酵真菌。需要进行物理化学特性、百分组成、木质纤维素和矿物质含量以及抗菌活性的分析。在不同的加湿条件下(水、无添加剂的营养溶液和含有葡萄糖或蔗糖的营养溶液)进行发酵 9 天。通过气相色谱法,辅助固相微萃取,定量生物香气。结果表明,用甜木薯进行发酵时,这种化合物的产量较低(约 6ppm(w/w))。甜木薯残渣生物产量低可能与其富含淀粉的组成、均匀的基质和低浓度的营养物质有关。已经使用了 buriti,但没有检测到香气的产生。可能是 buriti 中的硅和高木质素含量抑制了真菌的活性,使得难以获得所需的香气。鉴于废物的特点,选择了一种新的策略:以 1:1 的比例混合废物。使用添加葡萄糖的营养溶液进行发酵,产生了 156.24ppm(w/w)的香气。这种组合因此为该过程提供了更有利的环境,可能是由于甜木薯中的可发酵糖和 buriti 果皮中的脂肪酸的存在,从而证明了椰子香气生物产量增加约 2500%的可能性。