新冠病毒感染的肾移植患者的全面免疫分析
Comprehensive immune profiling of SARS-CoV-2 infected kidney transplant patients.
作者信息
Fenninger Franz, Sherwood Karen R, Wu Vivian, Wong Paaksum, DeMarco Mari L, Wang Meng, Benedicto Vincent, Dwarka Krishna A, Günther Oliver P, Tate Logan, Yoshida Eric, Keown Paul A, Kadatz Matthew, Lan James H
机构信息
Department of Medicine, University of British Columbia, Vancouver, BC, Canada.
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
出版信息
Front Transplant. 2023 Nov 20;2:1261023. doi: 10.3389/frtra.2023.1261023. eCollection 2023.
INTRODUCTION
The immune responses of kidney transplant recipients against SARS-CoV-2 remains under studied.
METHODS
In this prospective pilot study, we performed comprehensive immune profiling using cellular, proteomic, and serologic assays on a cohort of 9 kidney transplant recipients and 12 non-transplant individuals diagnosed with COVID-19.
RESULTS
Our data show that in addition to having reduced SARS-CoV-2 specific antibody levels, kidney transplant recipients exhibited significant cellular differences including a decrease in naïve-but increase in effector T cells, a high number of CD28+ CD4 effector memory T cells, and increased CD8 T memory stem cells compared with non-transplant patients. Furthermore, transplant patients had lower concentrations of serum cytokine MIP-1β as well as a less diverse T cell receptor repertoire.
CONCLUSION
Overall, our results show that compared to non-transplant patients, kidney transplant recipients with SARS-CoV-2 infection exhibit an immunophenotype that is reminiscent of the immune signature observed in patients with severe COVID-19.
引言
肾移植受者针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的免疫反应仍有待研究。
方法
在这项前瞻性试点研究中，我们对9名肾移植受者和12名被诊断为冠状病毒病2019（COVID-19）的非移植个体组成的队列进行了细胞、蛋白质组和血清学检测，以进行全面的免疫分析。
结果
我们的数据显示，除了严重急性呼吸综合征冠状病毒2特异性抗体水平降低外，与非移植患者相比，肾移植受者还表现出显著的细胞差异，包括初始T细胞减少但效应T细胞增加、大量CD28 + CD4效应记忆T细胞以及CD8 T记忆干细胞增加。此外，移植患者血清细胞因子巨噬细胞炎症蛋白1β（MIP-1β）浓度较低，且T细胞受体库的多样性较低。
结论
总体而言，我们的结果表明，与非移植患者相比，感染严重急性呼吸综合征冠状病毒2的肾移植受者表现出的免疫表型让人联想到在重症冠状病毒病2019患者中观察到的免疫特征。
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