用 C-甘氨酸或 C δ-氨基酮戊酸对血红素和卟啉进行放射性标记。
Radiolabeling Heme and Porphyrin with C-Glycine or C δ-Aminolevulinic Acid.
机构信息
Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA.
Department of Biological Sciences, University of Delaware, Newark, DE, USA.
出版信息
Methods Mol Biol. 2024;2839:225-231. doi: 10.1007/978-1-0716-4043-2_12.
PMID:39008256
Abstract
Radiolabeling enables the quantitation of newly synthesized heme and porphyrin, allowing us to distinguish heme synthesis rates from total cellular heme. Here, we describe a protocol for labeling heme with C-glycine or ALA and the sequential extraction of heme and porphyrin from the same samples for quantitation by liquid scintillation.
摘要
放射性标记使新合成的血红素和卟啉得以定量，使我们能够区分血红素合成率和细胞总血红素。在此，我们描述了一种用 C-甘氨酸或 ALA 标记血红素的方案，并从同一样品中顺序提取血红素和卟啉，通过液体闪烁进行定量。
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