中国鹦鹉热衣原体的一种新型变异株引起人类鹦鹉热。

A novel variant of Chlamydia psittaci causing human psittacosis in China.

机构信息

National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Department of Respiratory and Critical Care Medicine, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China.

出版信息

Int J Infect Dis. 2024 Oct;147:107180. doi: 10.1016/j.ijid.2024.107180. Epub 2024 Jul 25.

DOI:10.1016/j.ijid.2024.107180

PMID:39059574

Abstract

From January 2022 to November 2022, sporadic psittacosis occurred in Lishui city, China. The patients were presented with fever, cough, and pulmonary infiltration. Their clinical symptoms were not relieved after receiving cephalosporin, penicillin, beta-lactamase inhibitors, and quinolones. Metagenomic next-generation sequencing of bronchoalveolar lavage fluid samples from the patients revealed Chlamydia psittaci infection. Then, three C. psittaci strains were isolated from the patients. Their whole genome sequences (WGSs) were obtained, and a core genome multilocus sequence typing (cgMLST) method was developed to study the population structure of C. psittaci. Using the constructed cgMLST method, 72 WGSs were divided into four related groups and ten sub-clusters. The Lishui strains formed a unique population of C. psittaci, which might represent a new variant of C. psittaci. In vitro antimicrobial susceptibility testing suggested that the Lishui strains were sensitive to tetracycline, macrolides, quinolones, and no drug-resistance was observed.

摘要

从 2022 年 1 月到 2022 年 11 月，中国丽水市发生了散发性鹦鹉热。患者表现为发热、咳嗽和肺部浸润。他们在接受头孢菌素、青霉素、β-内酰胺酶抑制剂和喹诺酮类药物治疗后，临床症状并未缓解。对患者支气管肺泡灌洗液样本进行宏基因组下一代测序显示鹦鹉热衣原体感染。然后，从患者中分离出三株鹦鹉热衣原体。获得了它们的全基因组序列（WGS），并开发了核心基因组多位点序列分型（cgMLST）方法来研究鹦鹉热衣原体的种群结构。使用构建的 cgMLST 方法，将 72 个 WGS 分为四个相关组和十个亚群。丽水株形成了一个独特的鹦鹉热衣原体种群，可能代表了一种新的鹦鹉热衣原体变体。体外药敏试验表明，丽水株对四环素、大环内酯类、喹诺酮类敏感，未观察到耐药性。