Lahlou Aliénor, Coghill Ian, Davidson Mhairi L H, Billon Romain, Barneche Fredy, Lazar Dusan, Le Saux Thomas, Jullien Ludovic
PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, Paris, 75005, France.
Sony Computer Science Laboratories, Paris, 75005, France.
Adv Sci (Weinh). 2024 Sep;11(36):e2304420. doi: 10.1002/advs.202304420. Epub 2024 Jul 30.
Quantitative measurement of light intensity is a key step in ensuring the reliability and the reproducibility of scientific results in many fields of physics, biology, and chemistry. The protocols presented so far use various photoactive properties of manufactured materials. Here, leaves are introduced as an easily accessible green material to calibrate light intensity. The measurement protocol consists in monitoring the chlorophyll fluorescence of a leaf while it is exposed to a jump of constant light. The inverse of the characteristic time of the initial chlorophyll fluorescence rise is shown to be proportional to the light intensity received by the leaf over a wide range of wavelengths and intensities. Moreover, the proportionality factor is stable across a wide collection of plant species, which makes the measurement protocol accessible to users without prior calibration. This favorable feature is finally harnessed to calibrate a source of white light from exploiting simple leaves collected from a garden.
光强的定量测量是确保物理、生物和化学等许多领域科学结果的可靠性和可重复性的关键步骤。迄今为止提出的方案使用了人造材料的各种光活性特性。在这里,树叶被作为一种易于获取的绿色材料引入,用于校准光强。测量方案包括在叶片暴露于恒定光跃变时监测其叶绿素荧光。结果表明,初始叶绿素荧光上升的特征时间的倒数在很宽的波长和强度范围内与叶片接收到的光强成正比。此外,比例因子在广泛收集的植物物种中是稳定的,这使得该测量方案无需事先校准,用户即可使用。最后,利用这一有利特性,通过利用从花园采集的简单树叶来校准白光源。