韩国小型猪（Sus scrofa）的染色体水平基因组组装。

A chromosome-level genome assembly of the Korean minipig (Sus scrofa).

机构信息

Department of Biomedical Science and Engineering, Konkuk University, Seoul, 05029, Republic of Korea.

Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju, 55365, Republic of Korea.

出版信息

Sci Data. 2024 Aug 3;11(1):840. doi: 10.1038/s41597-024-03680-8.

DOI:10.1038/s41597-024-03680-8

PMID:39097649

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11297928/

Abstract

Recent advancements in sequencing and genome assembly technologies have led to rapid generation of high-quality genome assemblies for various species and breeds. Despite the importance as minipigs an animal model in biomedical research, the construction of high-quality genome assemblies of minipigs still lags behind other pig breeds. To address this problem, we constructed a high-quality chromosome-level genome assembly of the Korean minipig (KMP) utilizing multiple different types of sequencing reads and reference genomes. The KMP assembly included 19 chromosome-level sequences with a total length of 2.52 Gb and N50 of 137 Mb. Comparative analyses with the pig reference genome (Sscrofa11.1) demonstrated comparable contiguity and completeness of the KMP assembly. Additionally, genome annotation analyses identified 22,666 protein-coding genes and repetitive elements occupying 40.10% of the genome. The KMP assembly and genome annotation provide valuable resources that can contribute to various future research on minipig and other pig breeds.

摘要

近年来，测序和基因组组装技术的进步使得各种物种和品种的高质量基因组组装得以快速生成。尽管小型猪作为动物模型在生物医学研究中非常重要，但高质量的小型猪基因组组装仍然落后于其他猪品种。为了解决这个问题，我们利用多种不同类型的测序reads 和参考基因组，构建了韩国小型猪（KMP）的高质量染色体水平基因组组装。KMP 组装包括 19 条染色体水平的序列，总长度为 2.52Gb，N50 为 137Mb。与猪参考基因组（Sscrofa11.1）的比较分析表明，KMP 组装具有可比的连续性和完整性。此外，基因组注释分析鉴定了 22666 个蛋白质编码基因和重复元件，它们占据了基因组的 40.10%。KMP 组装和基因组注释提供了有价值的资源，可用于小型猪和其他猪品种的未来各种研究。