State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Houji Laboratory in Shanxi Province, Academy of Agronomy, Shanxi, China.
Plant Commun. 2024 Nov 11;5(11):101045. doi: 10.1016/j.xplc.2024.101045. Epub 2024 Aug 3.
The legume-rhizobium symbiosis represents the most important system for terrestrial biological nitrogen fixation on land. Efficient nitrogen fixation during this symbiosis depends on successful rhizobial infection and complete endosymbiosis, which are achieved by complex cellular events including cell-wall remodeling, cytoskeletal reorganizations, and extensive membrane expansion and trafficking. In this review, we explore the dynamic remodeling of the plant-specific cell wall-membrane system-cytoskeleton (WMC) continuum during symbiotic nitrogen fixation. We focus on key processes linked to efficient nitrogen fixation, including rhizobial uptake, infection thread formation and elongation, rhizobial droplet release, cytoplasmic bridge formation, and rhizobial endosymbiosis. Additionally, we discuss the advanced techniques for investigating the cellular basis of root-nodule symbiosis and provide insights into the unsolved mysteries of robust symbiotic nitrogen fixation.
豆科植物-根瘤菌共生代表了陆地生物固氮的最重要系统。在这种共生过程中，有效的氮固定取决于根瘤菌的成功感染和完全共生，这是通过包括细胞壁重塑、细胞骨架重组以及广泛的膜扩张和运输在内的复杂细胞事件来实现的。在这篇综述中，我们探讨了共生固氮过程中植物特有的细胞壁-膜系统-细胞骨架（WMC）连续体的动态重塑。我们专注于与有效氮固定相关的关键过程，包括根瘤菌的摄取、侵染线的形成和伸长、根瘤菌液滴的释放、细胞质桥的形成以及根瘤菌的内共生。此外，我们还讨论了研究根瘤共生细胞基础的先进技术，并深入了解了稳健共生固氮的未解之谜。
