Oral Health Prev Dent. 2024 Aug 6;22:381-388. doi: 10.3290/j.ohpd.b5656312.
To analyse the relative expression and diagnostic potential of lncRNA XIST (XIST) in peri-implantitis, and explore the related mechanism of XIST in peri-implantitis.
XIST expression in saliva of patients with peri-implantitis was detected by qRT-PCR. The diagnostic significance of XIST in peri-implantitis was assessed by ROC curve. Clinical indicators of the included patients were collected and the correlation between XIST levels and peri-implant indicators was determined by Pearson correlation analysis. Bioinformatic prediction and luciferase reporter assay confirmed the targeting relationship of XIST with downstream factors.
Salivary XIST levels were obviously higher in patients with peri-implantitis than in the healthy control group, and the AUC value for identifying patients was 0.8742 with a sensitivity and specificity of 83.5% and 81.4%. Patients in the peri-implantitis group had higher levels of plaque index (PLI), sulcus bleeding index (SBI) and probing depth (PD) than those in the healthy control group, and the expression of XIST was positively correlated with PLI, SBI, and PD levels. In addition, miR-150-5p was confirmed to be a potential downstream target of XIST.
XIST was overexpressed in the saliva of patients with peri-implantitis and correlated with the severity of the disease. XIST has high diagnostic significance for detecting peri-implantitis.
分析长链非编码 RNA XIST（XIST）在种植体周围炎中的相对表达量及其诊断潜力，并探讨 XIST 在种植体周围炎中的相关作用机制。
通过 qRT-PCR 检测种植体周围炎患者唾液中的 XIST 表达水平。采用 ROC 曲线评估 XIST 对种植体周围炎的诊断意义。收集纳入患者的临床指标，采用 Pearson 相关性分析确定 XIST 水平与种植体周围指标的相关性。通过生物信息学预测和荧光素酶报告基因实验验证 XIST 与下游因子的靶向关系。
种植体周围炎患者唾液中的 XIST 水平明显高于健康对照组，其鉴别患者的 AUC 值为 0.8742，灵敏度和特异度分别为 83.5%和 81.4%。种植体周围炎组患者的菌斑指数（PLI）、龈沟出血指数（SBI）和探诊深度（PD）均高于健康对照组，且 XIST 的表达与 PLI、SBI 和 PD 水平呈正相关。此外，miR-150-5p 被证实是 XIST 的潜在下游靶标。
XIST 在种植体周围炎患者的唾液中呈过表达，且与疾病的严重程度相关。XIST 对检测种植体周围炎具有较高的诊断意义。
