从中国分离的一株新型多耐药假单胞菌的特性研究，该菌含有染色体 bla 和一个携带 bla 和 bla 的巨大质粒。
Characterization of a novel multi-resistant Pseudomonas juntendi strain from China with chromosomal bla and a megaplasmid coharboring bla and bla.
机构信息
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, National Medical Center for Infectious Diseases, Zhejiang University School of Medicine, 79 Qingchun Rd, Hangzhou City, 310003, China.
Department Health Management Center, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
出版信息
BMC Genomics. 2024 Aug 8;25(1):774. doi: 10.1186/s12864-024-10688-2.
BACKGROUND
Pseudomonas juntendi is a newly identified opportunistic pathogen, of which we have limited understanding. P. juntendi strains are often multidrug resistant, which complicates clinical management of infection.
METHODS
A strain of Pseudomonas juntendi (strain L4326) isolated from feces was characterized by MALDI-TOF-MS and Average Nucleotide Identity BLAST. This strain was further subject to whole-genome sequencing and Maximum Likelihood phylogenetic analysis. The strain was phenotypically characterized by antimicrobial susceptibility testing and conjugation assays.
RESULTS
We have isolated the novel P. juntendi strain L4236, which was multidrug resistant, but retained sensitivity to amikacin. L4236 harbored a megaplasmid that encoded bla and a novel bla resistance gene variant. P. juntendi strain L4236 was phylogenetically related to P. juntendi strain SAMN30525517.
CONCLUSION
A rare P. juntendi strain was isolated from human feces in southern China with a megaplasmid coharboring bla and bla. Antimicrobial selection pressures may have driven acquisition of drug-resistance gene mutations and carriage of the megaplasmid.
背景
丛毛单胞菌是一种新发现的机会致病菌，我们对其了解有限。丛毛单胞菌菌株通常具有多重耐药性，这增加了感染的临床管理难度。
方法
从粪便中分离出一株丛毛单胞菌（菌株 L4326），通过 MALDI-TOF-MS 和平均核苷酸同一性 BLAST 进行鉴定。进一步对该菌株进行全基因组测序和最大似然系统发育分析。通过药敏试验和接合试验对该菌株进行表型特征分析。
结果
我们成功分离到一株新型丛毛单胞菌 L4236 菌株，该菌株具有多重耐药性，但对阿米卡星仍保持敏感性。L4236 携带有一个编码 bla 和一个新型 bla 耐药基因变异的大型质粒。丛毛单胞菌 L4236 菌株与丛毛单胞菌 SAMN30525517 菌株在系统发育上具有亲缘关系。
结论
在中国南方的人类粪便中分离到一株罕见的丛毛单胞菌 L4236 菌株，该菌株携带有一个大型质粒，同时携带 bla 和 bla 基因。抗菌药物选择压力可能导致耐药基因突变和大型质粒的携带。
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