Yang Jie, Zhuang Pingping, Cheng Ziqiang, Wang Guihua
Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
Vet Med (Praha). 2022 Feb 15;67(2):87-98. doi: 10.17221/141/2020-VETMED. eCollection 2022 Feb.
Exploration of the abnormal expression of exosomal molecules during the infection of avian leukosis virus subgroup J (ALV-J) is essential to provide a deeper understanding of the exosome's role in the viral pathogenesis involved. The study aimed to investigate the differentially expressed proteins and miRNAs of the exosomes derived from DF-1 cells infected by ALV-J, their gene function and involved signal pathways. We isolated exosomes from DF-1 cells infected by ALV-J. The differentially expressed proteins and miRNAs of the exosomes were determined by proteomics and transcription detection technology. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway analysis identified the miRNAs target genes and the signal pathways regulated by the different proteins or/and miRNAs. A total of 116 proteins (58 upregulated and 58 downregulated) and 3 miRNAs (all upregulated) were determined. These proteins were involved in 155 signal pathways, in which the highest number of proteins involved in the cancer pathway was (up to) seven. The target genes of the miRNAs were involved in 3 signal pathways. Both the proteins and target genes of the miRNAs were involved in the Ribosome pathway and ECM-receptor interaction pathway. The results suggested that the ALV-J infection changed the proteins and miRNAs of the exosomes significantly.
探索外泌体分子在禽白血病病毒J亚群（ALV-J）感染过程中的异常表达，对于更深入了解外泌体在相关病毒发病机制中的作用至关重要。本研究旨在调查受ALV-J感染的DF-1细胞来源的外泌体中差异表达的蛋白质和微小RNA（miRNA），及其基因功能和涉及的信号通路。我们从受ALV-J感染的DF-1细胞中分离出外泌体。通过蛋白质组学和转录检测技术确定外泌体中差异表达的蛋白质和miRNA。基因本体论（GO）分析和京都基因与基因组百科全书（KEGG）信号通路分析确定了miRNA的靶基因以及不同蛋白质或/和miRNA调控的信号通路。共确定了116种蛋白质（58种上调和58种下调）和3种miRNA（均上调）。这些蛋白质参与了155条信号通路，其中参与癌症通路的蛋白质数量最多（高达）7种。miRNA的靶基因参与了3条信号通路。miRNA的蛋白质和靶基因均参与核糖体通路和细胞外基质受体相互作用通路。结果表明，ALV-J感染显著改变了外泌体的蛋白质和miRNA。
