State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Genes (Basel). 2024 Jul 31;15(8):1001. doi: 10.3390/genes15081001.
Thiacloprid, a neonicotinoid pesticide, is known to affect the gut microbiome of honeybees, yet studies often focus on immediate alternations during exposure, overlooking long-term microbiological impacts post-exposure. This study investigates the influences of sublethal thiacloprid administered during the larval developmental stage of honeybees on physiological changes and gut microbiota of adult honeybees. We found that thiacloprid exposure increased mortality and sugar intake in emerged honeybees. Using 16S rDNA sequencing, we analyzed intestinal microbial diversity of honeybees at one and six days post-emergence. Our findings reveal a significant but transient disruption in gut microbiota on day 1, with recovery from dysbiosis by day 6. This study emphasizes the importance of evaluating chronic sublethal exposure risks of thiacloprid to protect honeybee health.
噻虫啉是一种新烟碱类杀虫剂，已知会影响蜜蜂的肠道微生物组，但研究通常集中在暴露期间的直接改变，而忽略了暴露后长期的微生物影响。本研究调查了在蜜蜂幼虫发育阶段给予亚致死剂量的噻虫啉对成年蜜蜂生理变化和肠道微生物组的影响。我们发现噻虫啉暴露会增加出房蜜蜂的死亡率和糖摄入量。使用 16S rDNA 测序，我们分析了出房后 1 天和 6 天的蜜蜂肠道微生物多样性。我们的研究结果表明，在第 1 天肠道微生物组出现了显著但短暂的紊乱，到第 6 天从菌群失调中恢复。这项研究强调了评估噻虫啉慢性亚致死暴露风险以保护蜜蜂健康的重要性。
