使用不溶性木聚糖底物测量木聚糖酶活性。
Measurement of xylanase activity with insoluble xylan substrate.
作者信息
Nummi M, Perrin J M, Niku-Paavola M L, Enari T M
出版信息
Biochem J. 1985 Mar 1;226(2):617-20. doi: 10.1042/bj2260617.
Abstract
Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.
摘要
通过碱提取和乙醇沉淀，从磨碎的白桦（毛桦）纸浆中制备了不溶性木聚糖。该制剂经酸水解后检测到的唯一糖类是木糖。在比浊法测定中，将不溶性木聚糖用作底物，以测定曲霉和木霉酶的木聚糖酶（EC 3.2.1.8，1,4-β-D-木聚糖木聚糖水解酶和EC 3.2.1.37，1,4-β-D-木聚糖木糖水解酶）活性。比浊法在评估不溶性木聚糖的木聚糖酶水解方面是可靠的。
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