微小RNA-19a-3p通过调控p53/SOX4促进肝细胞癌的生长。
miR-19a-3p promotes the growth of hepatocellular carcinoma by regulating p53/SOX4.
作者信息
Zhang Hang, Zhu Jiajun, Zhang Jingjun, Liu Ying, Zhao Baicheng, Yang Xiaoyi, Zhou Wenhan, Chen Bozhou, Zhang Shuangshuang, Huang Ruotong, Chen Shuying
机构信息
Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China.
Medical College, Fudan University, 130 Dongan Road, Shanghai, 200032, China.
出版信息
Heliyon. 2024 Aug 13;10(16):e36282. doi: 10.1016/j.heliyon.2024.e36282. eCollection 2024 Aug 30.
OBJECTIVE
This study aims to investigate the potential functions of miR-19a-3p in HCC.
METHOD
We collected serum samples to analyze miR-19a-3p expression. We utilized CCK8 and Transwell assays to access miR-19a-3p's influence on HCC cells malignancy. We used dual-luciferase reporter and western blotting to validate the impact of p53/miR-19 on miR-19/SOX4.
RESULTS
The results demonstrated that miR-19a-3p was highly expressed in pre-operative serum samples and HCC cells, which can promote cell proliferation, migration and invasion in HCC under conditions. Additionally, there was a p53 binding site on the upstream of miR-19a-3p, which was inhibited by p53. SOX4 was the direct gene targeted by miR-19a-3p. The imbalance of p53-miR-19-SOX4 loop was one reason for the progress of HCC.
CONCLUSION
Our findings validate the mechanisms of miR-19a-3p and highlight its potential as a therapeutic target in HCC.
目的
本研究旨在探讨miR-19a-3p在肝癌中的潜在功能。
方法
我们收集血清样本以分析miR-19a-3p的表达。我们利用CCK8和Transwell实验来评估miR-19a-3p对肝癌细胞恶性程度的影响。我们使用双荧光素酶报告基因和蛋白质免疫印迹法来验证p53/miR-19对miR-19/SOX4的影响。
结果
结果表明，miR-19a-3p在术前血清样本和肝癌细胞中高表达，在一定条件下可促进肝癌细胞的增殖、迁移和侵袭。此外，在miR-19a-3p上游存在一个p53结合位点，该位点受p53抑制。SOX4是miR-19a-3p的直接靶向基因。p53-miR-19-SOX4环的失衡是肝癌进展的原因之一。
结论
我们的研究结果验证了miR-19a-3p的作用机制，并突出了其作为肝癌治疗靶点的潜力。
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