炎症性肠病患者肠易激综合征和肠易激综合征样症状的多样本脂质组学特征：不同疾病中相同症状识别的新见解。
Multisample lipidomic profiles of irritable bowel syndrome and irritable bowel syndrome-like symptoms in patients with inflammatory bowel disease: new insight into the recognition of the same symptoms in different diseases.
机构信息
Department of Gastroenterology, China-Japan Friendship Hospital(Institute of Clinical Medical Sciences), Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100029, China.
School of Public Health, Capital Medical University, Beijing, 100069, China.
出版信息
J Gastroenterol. 2024 Nov;59(11):1000-1010. doi: 10.1007/s00535-024-02148-1. Epub 2024 Sep 10.
BACKGROUND
Overlapping clinical manifestations of irritable bowel syndrome (IBS) and IBS-like symptoms in patients with inflammatory bowel disease (IBD-IBS) present challenges in diagnosis and management. Both conditions are associated with alterations in metabolites, but few studies have described the lipid profiles. Our aim was to pinpoint specific lipids that contribute to the pathogenesis of IBS and IBD-IBS by analyzing multiple biologic samples.
METHODS
Diarrhea-predominant IBS (IBS-D) patients (n = 39), ulcerative colitis in remission with IBS-like symptoms patients (UCR-IBS) (n = 21), and healthy volunteers (n = 35) were recruited. IBS-D patients meet the Rome IV diagnostic criteria, and UCR-IBS patients matched mayo scores ≤ two points and Rome IV diagnostic criteria. Serum, feces, and mucosa were collected for further analysis. Lipid extraction was carried out by ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS).
RESULTS
Lipidomics of mucosa and serum samples significantly differed among the three groups. Feces showed the most altered lipid species, and the enrichment analysis of 347 differentially abundant metabolites via KEGG pathway analysis revealed that alpha-linolenic acid metabolism was significantly altered in the two groups (P < 0.01). The ratio of omega-6/omega-3 fatty acid were imbalance in serum samples.
CONCLUSIONS
This study revealed a comprehensive lipid composition pattern between IBS-D patients and UCR-IBS patients. We found several distinctive lipids involved in alpha-linolenic acid metabolism, reflecting an imbalance in the omega-6/omega-3 fatty acid ratio. Compared to mucosa and serum samples, fecal samples might have more advantages in lipidomics studies due to the convenience of sample collection and effectiveness in reflecting metabolic information.
背景
肠易激综合征（IBS）和炎症性肠病伴肠易激综合征（IBD-IBS）患者的临床表现重叠，这给诊断和治疗带来了挑战。这两种疾病都与代谢物的改变有关，但很少有研究描述过脂质谱。我们的目的是通过分析多种生物样本，确定导致 IBS 和 IBD-IBS 发病机制的特定脂质。
方法
招募了腹泻型肠易激综合征（IBS-D）患者（n=39）、缓解期伴有肠易激综合征样症状的溃疡性结肠炎患者（UCR-IBS）（n=21）和健康志愿者（n=35）。IBS-D 患者符合罗马 IV 诊断标准，UCR-IBS 患者符合 mayo 评分≤2 分和罗马 IV 诊断标准。收集血清、粪便和黏膜进行进一步分析。采用超高效液相色谱-高分辨质谱（UPLC-HRMS）进行脂质提取。
结果
三组黏膜和血清样本的脂质组学差异显著。粪便显示出最改变的脂质种类，通过 KEGG 途径分析对 347 种差异丰度代谢物的富集分析表明，两组的α-亚麻酸代谢明显改变（P<0.01）。血清样本中ω-6/ω-3 脂肪酸比例失衡。
结论
本研究揭示了 IBS-D 患者和 UCR-IBS 患者之间的综合脂质组成模式。我们发现了几种参与α-亚麻酸代谢的独特脂质，反映了 ω-6/ω-3 脂肪酸比例的失衡。与黏膜和血清样本相比，粪便样本在脂质组学研究中可能具有更多优势，因为样本采集方便，能够有效地反映代谢信息。
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