枯草芽孢杆菌温和噬菌体φ105粘性单链末端的核苷酸序列
Nucleotide sequence of the cohesive single-stranded ends of Bacillus subtilis temperate bacteriophage phi 105.
作者信息
Ellis D M, Dean D H
出版信息
J Virol. 1985 Aug;55(2):513-5. doi: 10.1128/JVI.55.2.513-515.1985.
Abstract
The cohesive single-stranded ends of temperate Bacillus subtilis phage phi 105 were analyzed with the exonuclease activities of the Klenow fragment of DNA polymerase I and with exonuclease III and were found to be 3' extensions. Chemical sequencing of 3'-end-labeled fragments showed that the ends are 7-base extended 3' single strands and have the sequence: 5'-GCGCTCC-3'. 3'-CGCGAGG-5'
摘要
利用DNA聚合酶I的Klenow片段的核酸外切酶活性以及核酸外切酶III对温和型枯草芽孢杆菌噬菌体phi 105的粘性单链末端进行了分析，结果发现它们是3'端延伸。对3'端标记片段的化学测序表明，末端是7个碱基延伸的3'单链，其序列为：5'-GCGCTCC-3'。3'-CGCGAGG-5'
Zotero 插件