Transcriptome-Based Screening of Candidate Low-Temperature-Associated Genes and Analysis of the Transcription Factor Gene Family in Kohlrabi ( L. var. L.).

Low temperature is a significant abiotic stress factor that not only impacts plant growth, development, yield, and quality but also constrains the geographical distribution of numerous wild plants. Kohlrabi ( L. var. L.) belongs to the Brassicaceae family and has a short growing period. In this study, a total of 196,642 unigenes were obtained from kohlrabi seedlings at low temperatures; of these, 52,836 unigenes were identified as differentially expressed genes. Transcription factor family members , , , etc. that had a high correlation with biochemical indicators related to low temperature were identified. A total of nineteen genes (named -) were obtained, and these genes were distributed unevenly across seven chromosomes. Nineteen genes searched four conserved motifs and were divided into three groups. The relative expression level analysis of 19 genes of kohlrabi showed obvious specificity in different tissues. This study lays a foundation and provides new insight to explain the low-temperature resistance mechanism and response pathways of kohlrabi. It also provides a theoretical basis for the functional analysis of 19 transcription factor gene family members.