花粉-食物过敏综合征：从食物回避到解析普李 3 与油橄榄 7 之间的潜在交叉反应性。

Pollen-Food Allergy Syndrome: From Food Avoidance to Deciphering the Potential Cross-Reactivity between Pru p 3 and Ole e 7.

机构信息

Department of Immunology and Allergy, Reina Sofía University Hospital, 14004 Córdoba, Spain.

Maimonides Biomedical Research Institute of Córdoba (IMIBIC)/Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, Spain.

出版信息

Nutrients. 2024 Aug 27;16(17):2869. doi: 10.3390/nu16172869.

DOI:10.3390/nu16172869

PMID:39275185

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11396898/

Abstract

BACKGROUND

Cross-reactivity between nonspecific lipid transfer proteins could cause anaphylaxis, further influencing food avoidance and nutrient deficiencies. The one affecting olive pollen (Ole e 7) and peach (Pru p 3) may underlie a variety of pollen-food syndromes, though a deep molecular analysis is necessary.

METHODS

Three Ole e 7-monosensitised patients (MON_OLE), three Pru p 3-monosensitised patients (MON_PRU) and three bisensitised patients (BI) were selected. For epitope mapping, both digested proteins were incubated with patient sera, and the captured IgE-bound peptides were characterised by LC-MS.

RESULTS

The analysis revealed two Ole e 7 epitopes and the three Pru p 3 epitopes previously described. Interestingly, the "KSALALVGNKV" Ole e 7 peptide was recognised by MON_OLE, BI and MON_PRU patients. Conversely, all patients recognised the "ISASTNCATVK" Pru p 3 peptide. Although complete sequence alignment between both proteins revealed 32.6% identity, local alignment considering seven residue fragments showed 50 and 57% identity when comparing "ISASTNCATVK" with Ole e 7 and "KSALALVGNKV" with Pru p 3.

CONCLUSIONS

This study mapped sIgE-Ole e 7-binding epitopes, paving the way for more precise diagnostic tools. Assuming non-significant sequence similarity, structural homology and shared key residues may underlie the potential cross-reactivity between Ole e 7 and Pru p 3 nsLTPs.

摘要

背景

非特异性脂质转移蛋白之间的交叉反应可能会引起过敏反应，进一步影响食物回避和营养缺乏。影响橄榄花粉（Ole e 7）和桃（Pru p 3）的蛋白可能是多种花粉-食物综合征的基础，但需要进行深入的分子分析。

方法

选择 3 名 Ole e 7 单敏患者（MON_OLE）、3 名 Pru p 3 单敏患者（MON_PRU）和 3 名双敏患者（BI）。为了进行表位作图，将两种消化蛋白与患者血清孵育，并通过 LC-MS 对捕获的 IgE 结合肽进行了表征。

结果

分析显示了两个 Ole e 7 表位和之前描述的三个 Pru p 3 表位。有趣的是，“KSALALVGNKV”Ole e 7 肽被 MON_OLE、BI 和 MON_PRU 患者识别。相反，所有患者都识别了“ISASTNCATVK”Pru p 3 肽。虽然两种蛋白的完整序列比对显示出 32.6%的同一性，但考虑到七个残基片段的局部比对显示，当比较“ISASTNCATVK”与 Ole e 7 和“KSALALVGNKV”与 Pru p 3 时，同一性分别为 50%和 57%。