Whole genome sequence data of ZEN-degrading strain PYN10_6_2 isolated from larval feces.

PYN10_6_2, a lactic acid bacterial strain previously isolated from larval feces, possesses the ability to convert zearalenone (ZEN) to α-/β-Zearalenol (α-/β-ZEL). However, the genes involved in the ZEN reduction reaction and the biosafety of this strain remain unknown. In this study, we sequenced, assembled, and annotated the whole genome of PYN10_6_2. Genomic sequencing was conducted using short-read sequencing on the Illumina HiSeq X Ten platform and long-read sequencing on the PacBio RS II Single Molecule Real-Time (SMRT) platform. The assembled genome consisted of one circular chromosome, four circular plasmids, with a total size of 2,745,725 bp and a G + C content of 45.52 %. Annotation identified 2,660 coding sequences, 5 rRNAs, 66 tRNAs, and a single CRISPR locus. Average nucleotide identity (ANI) between PYN10_6_2 and DSM 20054 yielded a value of 98.94 %. Further in-depth analysis revealed 182 antibiotic resistance genes, 237 putative virulence genes, 2 prophages, and 10 genomic islands. Additionally, functional annotation through COG and KEGG databases revealed the presence of three genes encoding 3α- and 3β-hydroxysteroid dehydrogenase (3α-/3β-HSD) within the bacterial chromosome. This comprehensive genomic characterization provides valuable insights into the genetic basis of PYN10_6_2's ZEN-reducing ability and its biosafety profile.