褪黑素与体育锻炼对肝硬化大鼠肝脏的作用：氧化应激与炎症过程的研究
Action of melatonin and physical exercise on the liver of cirrhotic rats: Study of oxidative stress and the inflammatory process.
作者信息
Martins Gabriela S, Rosa Carlos Gustavo S, Schemitt Elizangela G, Colares Josieli R, Fonseca Sandielly Rb, Brasil Marilda S, Engeroff Millena O, Marroni Norma P
机构信息
Department of Physiology, Faculty of Biological Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Experimental Laboratory of Pulmonology and Inflammation Sciences - Experimental Research Center, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre-RS, Brazil.
出版信息
Hepatol Forum. 2024 Sep 1;5(4):184-192. doi: 10.14744/hf.2023.2023.0037. eCollection 2024.
BACKGROUND AND AIM
Cirrhosis is characterized by structural and functional alterations of the liver. Melatonin (MLT) has antioxidant properties. Physical exercise (EX) can reverse muscle loss in cirrhotic patients. The objective was to evaluate the action of MLT and EX on the liver of rats subjected to the experimental model of bile duct ligation (BLD).
MATERIALS AND METHODS
48 male Wistar rats were used, divided into groups: Control (CO), CO+MLT, CO+EX, CO+MLT+EX, BDL, BDL+MLT, BDL+EX, and BDL+MLT+EX. The treatments occurred from the 15 to the 28 day. The dose of MLT was 20 mg/kg via I.p (1x/day), and the EX was performed 10 min/day. Blood and liver were collected for analysis.
RESULTS
The liver integrity enzymes AST, ALT, and ALP showed a significant reduction in the groups treated with MLT and EX. Histological analyses showed reorganization of the liver parenchyma, reduction of inflammatory infiltrate, and fibrotic nodules. Lipoperoxidation (LPO), the activity of antioxidant enzymes, and nitric oxide metabolites showed a significant reduction in the groups treated with MLT and EX. The expression of TNF-α and NF-kB decreased in the treated groups.
CONCLUSION
Melatonin and physical exercise seem to be effective in restoring the parameters evaluated in this model of experimental cirrhosis.
背景与目的
肝硬化的特征是肝脏的结构和功能改变。褪黑素（MLT）具有抗氧化特性。体育锻炼（EX）可逆转肝硬化患者的肌肉流失。目的是评估MLT和EX对胆管结扎（BLD）实验模型大鼠肝脏的作用。
材料与方法
使用48只雄性Wistar大鼠，分为以下几组：对照组（CO）、CO + MLT组、CO + EX组、CO + MLT + EX组、BDL组、BDL + MLT组、BDL + EX组和BDL + MLT + EX组。治疗从第15天持续到第28天。MLT的剂量为20 mg/kg，腹腔注射（每天1次），EX为每天进行10分钟。采集血液和肝脏进行分析。
结果
肝脏完整性酶AST、ALT和ALP在接受MLT和EX治疗的组中显著降低。组织学分析显示肝实质重组、炎症浸润减少和纤维化结节。脂质过氧化（LPO）、抗氧化酶活性和一氧化氮代谢产物在接受MLT和EX治疗的组中显著降低。治疗组中TNF-α和NF-κB的表达降低。
结论
褪黑素和体育锻炼似乎对恢复该实验性肝硬化模型中评估的参数有效。
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