全基因组 RNA-Seq 鉴定出 TP53 介导的胚胎干细胞抑制肿瘤侵袭和转移。
Genome-wide RNA-Seq identifies TP53-mediated embryonic stem cells inhibiting tumor invasion and metastasis.
机构信息
Department of Radiology, Peking Union Medical College Hospital, Beijing, 100730, China.
State Key Laboratory of Medical Molecular Biology, Department of Pathology and Pathophysiology, Institute of Basic Medical Sciences, Center of Molecular Pathology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100005, China.
出版信息
Stem Cell Res Ther. 2024 Oct 16;15(1):369. doi: 10.1186/s13287-024-04000-y.
The discovery of embryonic stem cell (ESC) mediating tumoricidal activity revealed the intimate relationship between ESCs and tumor cells, but the functional role of ESCs in tumor progression is poorly understood. To further investigate tumor cell and ESC interactions, we co-cultured mouse ESCs with mouse pancreatic cancer Pan02 cells or mouse melanoma B16-F10 cells in Transwell, and found that tumor cell invasion was significantly inhibited by ESCs. Application of ESCs to tumor-bearing mice resulted in significant inhibition of tumor metastasis in vivo. RNA-Seq analyses of tumor cell and ESC co-cultures identified TP53 and related signalling as major pathways involved in ESC-mediated inhibition of tumor cell invasion and metastasis, which indicated the potential clinical application of ESCs to treat cancer.
胚胎干细胞（ESC）介导的杀瘤活性的发现揭示了 ESC 和肿瘤细胞之间的密切关系，但 ESC 在肿瘤进展中的功能作用尚不清楚。为了进一步研究肿瘤细胞和 ESC 的相互作用，我们将小鼠 ESC 与小鼠胰腺癌 Pan02 细胞或小鼠黑色素瘤 B16-F10 细胞共培养于 Transwell 中，发现 ESC 显著抑制肿瘤细胞侵袭。将 ESC 应用于荷瘤小鼠，导致体内肿瘤转移的显著抑制。肿瘤细胞和 ESC 共培养的 RNA-Seq 分析鉴定出 TP53 及相关信号通路是 ESC 抑制肿瘤细胞侵袭和转移的主要途径，这表明 ESC 具有治疗癌症的潜在临床应用价值。
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