Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Redox Biol. 2024 Nov;77:103402. doi: 10.1016/j.redox.2024.103402. Epub 2024 Oct 16.
Oxidative stress is a crucial concept in redox biology, and significant progress has been made in recent years. Excessive levels of reactive oxygen species (ROS) can lead to oxidative damage, heightening vulnerability to various diseases. By contrast, ROS maintained within a moderate range plays a role in regulating normal physiological metabolism. Choosing suitable animal models in a complex research context is critical for enhancing research efficacy. While rodents are frequently utilized in medical experiments, they pose challenges such as high costs and ethical considerations. Alternatively, non-rodent model organisms like zebrafish, Drosophila, and C. elegans offer promising avenues into oxidative stress research. These organisms boast advantages such as their small size, high reproduction rate, availability for live imaging, and ease of gene manipulation. This review highlights advancements in the detection of oxidative stress using non-rodent models. The oxidative homeostasis regulatory pathway, Kelch-like ECH-associated protein 1-Nuclear factor erythroid 2-related factor 2 (Keap1-Nrf2), is systematically reviewed alongside multiple regulation of Nrf2-centered pathways in different organisms. Ultimately, this review conducts a comprehensive comparative analysis of different model organisms and further explores the combination of novel techniques with non-rodents. This review aims to summarize state-of-the-art findings in oxidative stress research using non-rodents and to delineate future directions.
氧化应激是氧化还原生物学中的一个关键概念，近年来取得了重大进展。过量的活性氧物种（ROS）会导致氧化损伤，增加患各种疾病的脆弱性。相比之下，维持在适度范围内的 ROS 则在调节正常生理代谢中发挥作用。在复杂的研究背景下，选择合适的动物模型对于提高研究效果至关重要。虽然啮齿动物常用于医学实验，但它们存在成本高和伦理问题等挑战。相比之下，斑马鱼、果蝇和秀丽隐杆线虫等非啮齿类模式生物为氧化应激研究提供了有前途的途径。这些生物体具有体型小、繁殖率高、可进行活体成像和易于基因操作等优势。本文综述了使用非啮齿类模型检测氧化应激的进展。系统综述了氧化平衡调节途径 Kelch-like ECH-associated protein 1-Nuclear factor erythroid 2-related factor 2 (Keap1-Nrf2)，以及不同生物体中 Nrf2 为中心的多种途径的调控。最终，本文对不同模型生物体进行了全面的比较分析，并进一步探讨了将新型技术与非啮齿类动物结合的应用。本文旨在总结使用非啮齿类动物进行氧化应激研究的最新发现，并描绘未来的方向。
