Ticer Taylor D, Tingler Anna M, Glover Janiece S, Dooley Sarah A, Kendrick Jacob, Zackular Joseph P, Devkota Suzanne, Wu Gary D, Mahalak Karley, Engevik Amy, Engevik Melinda A
Department of Microbiology & Immunology, Medical University of South Carolina, Charleston, SC, United States.
Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC, United States.
Front Microbiol. 2024 Oct 8;15:1459795. doi: 10.3389/fmicb.2024.1459795. eCollection 2024.
is a bacterial pathogen that has been implicated in severe gastrointestinal infections. has intrinsic green autofluorescence and the level of this autofluorescence is known to be increased by growth time and oxygen. Currently, it is unclear if dietary compounds or metabolites from the gut microbiota are able to enhance autofluorescence. Here, we aimed to determine potential factors that affect autofluorescence. After screening a large repertoire of compounds, we identified several substances, like L-lysine and pantothenate, that led to an increased autofluorescence. We also found that several members of the gut microbiota, such as and , can increase autofluorescence through their secreted compounds. We further focused on the effect of on autofluorescence and found that multiple enteric strains of could enhance autofluorescence. We used this enhanced autofluorescence to identify in co-cultures by flow cytometry. Our findings shed light on the relationship between and other members of the gut microbiota, as well as different factors that can affect autofluorescence.
是一种与严重胃肠道感染有关的细菌病原体。具有内在的绿色自发荧光，并且已知这种自发荧光的水平会随着生长时间和氧气而增加。目前，尚不清楚来自肠道微生物群的膳食化合物或代谢产物是否能够增强自发荧光。在这里，我们旨在确定影响自发荧光的潜在因素。在筛选了大量化合物之后，我们鉴定出了几种物质，如L-赖氨酸和泛酸盐，它们导致自发荧光增加。我们还发现肠道微生物群的几个成员，如和，可以通过它们分泌的化合物增加自发荧光。我们进一步关注对自发荧光的影响，发现多种肠道菌株可以增强自发荧光。我们利用这种增强的自发荧光通过流式细胞术在共培养物中鉴定。我们的研究结果揭示了与肠道微生物群其他成员之间的关系，以及可以影响自发荧光的不同因素。
