重要作物钙调素结合蛋白 60 候选基因的全基因组分析。

Genome-wide analysis of calmodulin binding Protein60 candidates in the important crop plants.

机构信息

Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.

Department of Agricultural Biotechnology & Molecular Biology, College of Basic Sciences & Humanities, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, India.

出版信息

Mol Biol Rep. 2024 Oct 30;51(1):1105. doi: 10.1007/s11033-024-10032-7.

DOI:10.1007/s11033-024-10032-7

PMID:39476040

Abstract

BACKGROUND

Efficient management of environmental stresses is essential for sustainable crop production. Calcium (Ca²⁺) signaling plays a crucial role in regulating responses to both biotic and abiotic stresses, particularly during host-pathogen interactions. In Arabidopsis thaliana, calmodulin-binding protein 60 (CBP60) family members, such as AtCBP60g, AtCBP60a, and AtSARD1, have been well characterized for their involvement in immune regulation. However, a comprehensive understanding of CBP60 genes in major crops remains limited.

METHODS

In this study, we utilized the Phytozome v12.1 database to identify and analyze CBP60 genes in agriculturally important crops. Expression patterns of a Oryza sativa (rice) CBP60 gene, OsCBP60bcd-1, were assessed in resistant and susceptible rice genotypes in response to infection by the bacterial pathogen Xanthomonas oryzae. Localization of CBP60 proteins was analyzed to predict their functional roles, and computational promoter analysis was performed to identify stress-responsive cis-regulatory elements.

RESULTS

Phylogenetic analysis revealed that most CBP60 genes in crops belong to the immune-related clade. Expression analysis showed that OsCBP60bcd-1 was significantly upregulated in the resistant rice genotype upon pathogen infection. Subcellular localization studies suggested that the majority of CBP60 proteins are nuclear-localized, indicating a potential role as transcription factors. Promoter analysis identified diverse stress-responsive cis-regulatory elements in the promoters of CBP60 genes, highlighting their regulatory potential under stress conditions.

CONCLUSION

The upregulation of OsCBP60bcd-1 in response to Xanthomonas oryzae and the presence of stress-responsive elements in its promoter underscore the importance of CBP60 genes in pathogen defense. These findings provide a basis for further investigation into the functional roles of CBP60 genes in crop disease resistance, with implications for enhancing stress resilience in agricultural species.

摘要

背景

有效的环境压力管理对于可持续的作物生产至关重要。钙（Ca²⁺）信号在调节生物和非生物胁迫的反应中起着至关重要的作用，特别是在宿主-病原体相互作用期间。在拟南芥中，钙调素结合蛋白 60（CBP60）家族成员，如 AtCBP60g、AtCBP60a 和 AtSARD1，已经被很好地描述为参与免疫调节。然而，对主要作物中 CBP60 基因的全面了解仍然有限。

方法

在这项研究中，我们利用 Phytozome v12.1 数据库鉴定和分析了农业上重要作物中的 CBP60 基因。评估了水稻 CBP60 基因 OsCBP60bcd-1 在对细菌性病原体稻黄单胞菌感染的抗性和感病水稻基因型中的表达模式。分析 CBP60 蛋白的定位以预测其功能作用，并进行计算启动子分析以鉴定应激响应的顺式调控元件。

结果

系统发育分析表明，作物中的大多数 CBP60 基因属于与免疫相关的分支。表达分析表明，OsCBP60bcd-1 在抗性水稻基因型受到病原体感染时显著上调。亚细胞定位研究表明，大多数 CBP60 蛋白定位于核内，表明其作为转录因子的潜在作用。启动子分析鉴定了 CBP60 基因启动子中多种应激响应的顺式调控元件，突出了它们在应激条件下的调节潜力。